Table 1.

Primer sequences

Primer5′ → 3′ Sequence
Genotyping primers 
 Lman1 primer A GGC​TTT​CTT​GAC​ACC​TTC​AAT​TTA​A 
 Lman1 primer B CCA​AGT​GAA​GGG​AAG​ACC​ATC​AAG​C 
 Lman1 primer C GAC​CCC​TAG​TGA​CGG​GTT​CTT​GTC 
 EIIA-Cre transgene Fwd CCG​CTG​GAG​ATG​ACG​TAG​TT 
 EIIA-Cre transgene Rev CGC​ATA​ACC​AGT​GAA​ACA​GCA​TTG​C 
qRT-PCR primers 
 Gapdh Fwd TGT​GTC​CGT​CGT​GGA​TCT​GA 
 Gapdh Rev ACC​ACC​TTC​TTG​ATG​TCA​TCA​TAC​TT 
 β-actin Fwd CTA​AGG​CCA​ACC​GTG​AAA​AG 
 β-actin Rev GGG​GTG​TTG​AAG​GTC​TCA​AA 
 F5 ACG​TGG​TGA​AGA​GAG​ATA​TCG​AC 
 R5 ACA​AAG​TGG​ATC​GTG​GAT​AGA​CA 
 F6 GCC​CAG​GCG​GGG​AAT​GCT​ATT​CCA​AG 
 R6 TCT​CGA​AGG​CTG​CTT​TTG​CTT​TGG​T 
 Aft6 Fwd CTT​CCT​CCA​GTT​GCT​CCA​TC 
 Aft6 Rev CAA​CTC​CTC​AGG​AAC​GTG​CT 
 BiP Fwd CAT​GGT​TCT​CAC​TAA​AAT​GAA​AGG 
 BiP Rev GCT​GGT​ACA​GTA​ACA​ACT​G 
 Chop Fwd CTG​CCT​TTC​ACC​TTG​GAG​AC 
 Chop Rev CGT​TTC​CTG​GGG​ATG​AGA​TA 
 XBP total Fwd AAG​AAC​ACG​CTT​GGG​AAT​GG 
 XBP total Rev ACTCCCCTTGGCCTCCAC 
 XBP spliced Fwd GAGTCCGCAGCAGGTG 
 XBP spliced Rev GTGTCAGAGTCCATGGGA 
Primer5′ → 3′ Sequence
Genotyping primers 
 Lman1 primer A GGC​TTT​CTT​GAC​ACC​TTC​AAT​TTA​A 
 Lman1 primer B CCA​AGT​GAA​GGG​AAG​ACC​ATC​AAG​C 
 Lman1 primer C GAC​CCC​TAG​TGA​CGG​GTT​CTT​GTC 
 EIIA-Cre transgene Fwd CCG​CTG​GAG​ATG​ACG​TAG​TT 
 EIIA-Cre transgene Rev CGC​ATA​ACC​AGT​GAA​ACA​GCA​TTG​C 
qRT-PCR primers 
 Gapdh Fwd TGT​GTC​CGT​CGT​GGA​TCT​GA 
 Gapdh Rev ACC​ACC​TTC​TTG​ATG​TCA​TCA​TAC​TT 
 β-actin Fwd CTA​AGG​CCA​ACC​GTG​AAA​AG 
 β-actin Rev GGG​GTG​TTG​AAG​GTC​TCA​AA 
 F5 ACG​TGG​TGA​AGA​GAG​ATA​TCG​AC 
 R5 ACA​AAG​TGG​ATC​GTG​GAT​AGA​CA 
 F6 GCC​CAG​GCG​GGG​AAT​GCT​ATT​CCA​AG 
 R6 TCT​CGA​AGG​CTG​CTT​TTG​CTT​TGG​T 
 Aft6 Fwd CTT​CCT​CCA​GTT​GCT​CCA​TC 
 Aft6 Rev CAA​CTC​CTC​AGG​AAC​GTG​CT 
 BiP Fwd CAT​GGT​TCT​CAC​TAA​AAT​GAA​AGG 
 BiP Rev GCT​GGT​ACA​GTA​ACA​ACT​G 
 Chop Fwd CTG​CCT​TTC​ACC​TTG​GAG​AC 
 Chop Rev CGT​TTC​CTG​GGG​ATG​AGA​TA 
 XBP total Fwd AAG​AAC​ACG​CTT​GGG​AAT​GG 
 XBP total Rev ACTCCCCTTGGCCTCCAC 
 XBP spliced Fwd GAGTCCGCAGCAGGTG 
 XBP spliced Rev GTGTCAGAGTCCATGGGA 
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