Histologic analysis of testicular tissue samples retrieved from prepubertal patients with SCD in the context of fertility preservation according to exposure to hydroxyurea
| . | Exposed to HU . | Not exposed to HU . | P . | |||
|---|---|---|---|---|---|---|
| Off-HU . | On-HU . | Total . | P1 . | P2 . | ||
| Patients, n | 11 | 6 | 17 | 13 | ||
| Samples, n | 17 | 8 | 25 | 23 | ||
| Treatment characteristics | ||||||
| Age at HU onset, median (range), y | 5.2 (3.4-10.9) | 4.2 (2.2-9.0) | 5.0 (2.2-10.9) | — | — | .33 |
| HU dosing, median (range), mg/kg/d | 22.2 ± 4.0 (15-28) | 22.5 ± 2.7 (20-25) | 22.0 (15-28) | — | — | .91 |
| HU time of exposure, median (range), mo | 27.6 (8.0-67.0) | 37.5 (9.5-66.0) | 36.0 (8.0-67.0) | — | — | .80 |
| Washout period, median (range), mo | 5.2 (2.2-36.9) | 0.0 | 3.0 (0.0-36.9) | — | — | — |
| Transfusion therapy, median (range), mo | 24.9 (2.4-71.4)$ | 13.2 (3.0-36.0)* | 15.4 (2.4-71.4) | 15.4 (0.9-42.8)† | .70 | .62 |
| ITT histologic analysis | ||||||
| Age at ITT-CP, median (range), y | 10.1 (5.8-15) | 7.9 (4.2-11.3) | 8.8 (4.2-15.0) | 8.0 (4.2-11.8) | .39 | .42 |
| Cross-sections analyzed, mean ± SD (range), n | 77 ± 49 (10-165) | 102 ± 33 (60-143) | 85 ± 45 (10-165) | 87 ± 31 (51-148) | .9 | .26 |
| S/T ratio, mean ± SD (range) | 3.1 ± 4.0 (0.6-14.0) | 1.5 ± 0.7 (0.8-2.4) | 2.5 ± 3.3 (0.6-14.0) | 1.7 ± 0.6 (0.7-2.7) | .61 | .84 |
| SCO tubules, mean ± SD (range), % | 44 ± 23 (0-77) | 40 ± 17 (18-55) | 42 ± 21 (0-77) | 38 ± 16 | .52 | .72 |
| . | Exposed to HU . | Not exposed to HU . | P . | |||
|---|---|---|---|---|---|---|
| Off-HU . | On-HU . | Total . | P1 . | P2 . | ||
| Patients, n | 11 | 6 | 17 | 13 | ||
| Samples, n | 17 | 8 | 25 | 23 | ||
| Treatment characteristics | ||||||
| Age at HU onset, median (range), y | 5.2 (3.4-10.9) | 4.2 (2.2-9.0) | 5.0 (2.2-10.9) | — | — | .33 |
| HU dosing, median (range), mg/kg/d | 22.2 ± 4.0 (15-28) | 22.5 ± 2.7 (20-25) | 22.0 (15-28) | — | — | .91 |
| HU time of exposure, median (range), mo | 27.6 (8.0-67.0) | 37.5 (9.5-66.0) | 36.0 (8.0-67.0) | — | — | .80 |
| Washout period, median (range), mo | 5.2 (2.2-36.9) | 0.0 | 3.0 (0.0-36.9) | — | — | — |
| Transfusion therapy, median (range), mo | 24.9 (2.4-71.4)$ | 13.2 (3.0-36.0)* | 15.4 (2.4-71.4) | 15.4 (0.9-42.8)† | .70 | .62 |
| ITT histologic analysis | ||||||
| Age at ITT-CP, median (range), y | 10.1 (5.8-15) | 7.9 (4.2-11.3) | 8.8 (4.2-15.0) | 8.0 (4.2-11.8) | .39 | .42 |
| Cross-sections analyzed, mean ± SD (range), n | 77 ± 49 (10-165) | 102 ± 33 (60-143) | 85 ± 45 (10-165) | 87 ± 31 (51-148) | .9 | .26 |
| S/T ratio, mean ± SD (range) | 3.1 ± 4.0 (0.6-14.0) | 1.5 ± 0.7 (0.8-2.4) | 2.5 ± 3.3 (0.6-14.0) | 1.7 ± 0.6 (0.7-2.7) | .61 | .84 |
| SCO tubules, mean ± SD (range), % | 44 ± 23 (0-77) | 40 ± 17 (18-55) | 42 ± 21 (0-77) | 38 ± 16 | .52 | .72 |
In the comparison of HU-exposed and nonexposed groups, patients with bilateral surgery account for a single value (see supplemental Materials and Methods). P1, comparison of values between HU-exposed and nonexposed patients, Student t test; P2, comparison of values between off-HU and on-HU patients, Mann-Whitney test.
One missing data point.
Two missing data points.