List of initial workup studies for patients presenting with a possible diagnosis of pure red cell aplasia
| Studies . |
|---|
| Anemia |
| CBC with differential |
| Reticulocyte count |
| EPO levels |
| Coombs test |
| Nutritional deficiencies |
| Hb electrophoresis and peripheral blood smear examination |
| Diamond-Blackfan anemia |
| Erythrocyte ADA (before transfusion of red cells) |
| Genetic testing |
| LGL |
| Flow cytometry (as part of a low-grade lymphoma panel) |
| TCR rearrangement |
| STAT3 gene mutations (eg, in order of frequency Y640F, D661Y, D661V, N647I) |
| Autoimmunity and immunologic conditions |
| Antinuclear antibodies |
| Rheumatoid arthritis antibodies |
| Extractable nuclear antigen panel |
| Lymphocyte subsets (flow cytometry) |
| B-cell dyscrasias studies |
| Serum electrophoresis |
| Serum immunofixation |
| Free light chains |
| Immunoglobulins levels |
| Bone marrow |
| Morphology (myeloid:erythroid ratio, dysplastic changes, giant pronormoblasts) |
| Flow cytometry for LGL |
| Cytogenetics |
| TCR rearrangement |
| Virological |
| B19 parvovirus |
| Viral hepatitis |
| HIV |
| EBV |
| CMV |
| Anamnestic data about drug history |
| Thymoma studies* |
| CT scan of the chest |
| Studies . |
|---|
| Anemia |
| CBC with differential |
| Reticulocyte count |
| EPO levels |
| Coombs test |
| Nutritional deficiencies |
| Hb electrophoresis and peripheral blood smear examination |
| Diamond-Blackfan anemia |
| Erythrocyte ADA (before transfusion of red cells) |
| Genetic testing |
| LGL |
| Flow cytometry (as part of a low-grade lymphoma panel) |
| TCR rearrangement |
| STAT3 gene mutations (eg, in order of frequency Y640F, D661Y, D661V, N647I) |
| Autoimmunity and immunologic conditions |
| Antinuclear antibodies |
| Rheumatoid arthritis antibodies |
| Extractable nuclear antigen panel |
| Lymphocyte subsets (flow cytometry) |
| B-cell dyscrasias studies |
| Serum electrophoresis |
| Serum immunofixation |
| Free light chains |
| Immunoglobulins levels |
| Bone marrow |
| Morphology (myeloid:erythroid ratio, dysplastic changes, giant pronormoblasts) |
| Flow cytometry for LGL |
| Cytogenetics |
| TCR rearrangement |
| Virological |
| B19 parvovirus |
| Viral hepatitis |
| HIV |
| EBV |
| CMV |
| Anamnestic data about drug history |
| Thymoma studies* |
| CT scan of the chest |
Particulary in case of history of myasthenia gravis.
ADA, adenosine deaminase; EBV: Epstein-Barr virus; EPO, erythropoietin; STAT3, signal transducer and activator of transcription 3.