Table 1.

List of sgRNA/shRNA target sequences for validation of CRISPR screen hits

Target namesgRNA/shRNA target sequences
sgGFP GAG​ATC​GAG​TGC​CGC​ATC​AC 
sgControl GCC​CGT​TTC​GTC​ATT​CCC​AC 
sgAAVS ATT​CCC​AGG​GCC​GGT​TAA​TG 
sgATXN7L3-1 ACA​GCA​CAC​AGA​TGA​GCA​G 
sgATXN7L3-2 TTG​TTG​GAA​CCT​AGG​CCG​G 
sgENY2-1 GTG​GCT​GAA​ATC​ACT​CCA​AA 
sgENY2-2 ACA​CGT​TAC​TGT​TGA​TGA​CT 
sgUSP22-1 AGA​CAT​GGA​AAT​AAT​CGC​CA 
sgUSP22-2 AAG​GCG​AAG​CGG​CAC​AAC​CT 
sgFCGR1A-1 GAC​ATC​CCC​ACT​CCT​GGA​G 
sgFCGR1A-2 TGG​GAG​CAG​CTC​TAC​ACA​G 
shControl CGA​GGG​CGA​CTT​AAC​CTT​AGG 
shUSP22-1 CCT​ACC​TGC​TGT​AAG​ATT​ATG 
shUSP22-2 AGC​TAC​CAG​GAG​TCC​ACA​AAG 
shENY2-1 CCA​GCC​TTT​AAG​ATT​GAA​TTA 
shENY2-2 GCA​CAC​TGT​AAA​GAG​GTA​ATT 
shATXN7L3-1 AGG​CGA​ACC​GTA​CGG​ATT​TAT 
shATXN7L3-2 GCA​GGC​AGA​GGA​TCC​GAT​TAT 
Target namesgRNA/shRNA target sequences
sgGFP GAG​ATC​GAG​TGC​CGC​ATC​AC 
sgControl GCC​CGT​TTC​GTC​ATT​CCC​AC 
sgAAVS ATT​CCC​AGG​GCC​GGT​TAA​TG 
sgATXN7L3-1 ACA​GCA​CAC​AGA​TGA​GCA​G 
sgATXN7L3-2 TTG​TTG​GAA​CCT​AGG​CCG​G 
sgENY2-1 GTG​GCT​GAA​ATC​ACT​CCA​AA 
sgENY2-2 ACA​CGT​TAC​TGT​TGA​TGA​CT 
sgUSP22-1 AGA​CAT​GGA​AAT​AAT​CGC​CA 
sgUSP22-2 AAG​GCG​AAG​CGG​CAC​AAC​CT 
sgFCGR1A-1 GAC​ATC​CCC​ACT​CCT​GGA​G 
sgFCGR1A-2 TGG​GAG​CAG​CTC​TAC​ACA​G 
shControl CGA​GGG​CGA​CTT​AAC​CTT​AGG 
shUSP22-1 CCT​ACC​TGC​TGT​AAG​ATT​ATG 
shUSP22-2 AGC​TAC​CAG​GAG​TCC​ACA​AAG 
shENY2-1 CCA​GCC​TTT​AAG​ATT​GAA​TTA 
shENY2-2 GCA​CAC​TGT​AAA​GAG​GTA​ATT 
shATXN7L3-1 AGG​CGA​ACC​GTA​CGG​ATT​TAT 
shATXN7L3-2 GCA​GGC​AGA​GGA​TCC​GAT​TAT 

Multiple targets and their respective sequences based on the results from the CRISPR screen are shown (Figures 2 and 3). sgRNAs were validated in Cas9-expressing AML2 and AML3 cell lines or AML2 and AML3 cells.

shRNA, short hairpin RNA.

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