Genotyping of mouse embryos at E12.5
| . | Rpl5+/+ . | Rpl5+/− . | Total . | % Rpl5+/− . |
|---|---|---|---|---|
| Genotyped live embryos (n = 28) | ||||
| Observed | 15 | 13 | 28 | 46 |
| Predicted | 14 | 14 | 28 | 50 |
| . | Rpl5+/+ . | Rpl5+/− . | Total . | % Rpl5+/− . |
|---|---|---|---|---|
| Genotyped live embryos (n = 28) | ||||
| Observed | 15 | 13 | 28 | 46 |
| Predicted | 14 | 14 | 28 | 50 |
Timed matings were set up with WT females x Rpl5+/− male mice. Pregnant females were killed at E12.5, and genotyping was performed on all embryos. Genotyping performed on 5 litters (n = 38) revealed 28 live embryos with no significant difference in observed to expected Mendelian ratio (χ2 analysis, P = 0.7). An additional 10 Rpl5+/− embryos (26%) were found to be dead or necrotic (dead embryos not included in the Mendelian ratio analysis ).