Procedures for vitreous sampling and analysis
| Before sampling . | Technique for sampling . | Eye samples . | Analysis of sample . | |
|---|---|---|---|---|
| Discussion with the pathologist for prompt processing of the sample | Gentle diagnostic vitrectomy (low cutting speed) | Pure vitreous | Cytology Immunocytochemistry, flow cytometry Preferably without a fixative and processed within 2 h after the reception; if a fixative is required, HOPE fixation is preferred | At least 4 cytospins 2 cytospots for cytomorphological evaluation after MGG coloration 2 cytospots for immunocytochemistry Use the total amount of available material |
| Cytokines (IL-10 and IL-6) without a fixative | Storage at −80°C Measure of cytokine levels in thawed samples | |||
| Vitreous diluted in BSS during the surgical procedure | Preferably without a fixative and processed within 2 h after the reception; if fixative is required, HOPE fixation is preferred | |||
| Immunocytochemistry, flow cytometry, molecular biology | 2 cytospots for cytomorphological evaluation after MGG coloration 2 cytospots for immunocytochemistry Remaining slides stored at −30°C | |||
| Before sampling . | Technique for sampling . | Eye samples . | Analysis of sample . | |
|---|---|---|---|---|
| Discussion with the pathologist for prompt processing of the sample | Gentle diagnostic vitrectomy (low cutting speed) | Pure vitreous | Cytology Immunocytochemistry, flow cytometry Preferably without a fixative and processed within 2 h after the reception; if a fixative is required, HOPE fixation is preferred | At least 4 cytospins 2 cytospots for cytomorphological evaluation after MGG coloration 2 cytospots for immunocytochemistry Use the total amount of available material |
| Cytokines (IL-10 and IL-6) without a fixative | Storage at −80°C Measure of cytokine levels in thawed samples | |||
| Vitreous diluted in BSS during the surgical procedure | Preferably without a fixative and processed within 2 h after the reception; if fixative is required, HOPE fixation is preferred | |||
| Immunocytochemistry, flow cytometry, molecular biology | 2 cytospots for cytomorphological evaluation after MGG coloration 2 cytospots for immunocytochemistry Remaining slides stored at −30°C | |||
Data adapted.24,27,99
BSS, balanced salt solution; HOPE, herpes Gglutamic acid buffer-mediated organic solvent protection effect; MGG, May-Grünwald-Giemsa.