Summary of cell line killing mediated by WT1-TCB
| Cell line . | Disease of tissue origin . | WT1 mRNA . | HLA-A typing based on TRON database . | Time of EC50 evaluation (h) . | EC50 (nM) T-cell killing in vitro . | EC50 (µg/mL) T-cell killing in vitro . |
|---|---|---|---|---|---|---|
| SKM-1 | AML | + | 33:03, 02:07 | 44 | 0.16-1.25 | 0.032-0.25 |
| OCI-M1 | AML | + | 03:01, 02:01 | 68 | 0.75-2.15 | 0.15-0.43 |
| OCI-AML3 | AML | + | 23:01, 02:01 | 68 | 0.20-6.75 | 0.04-1.35 |
| Set-2 | AML | + | 33:03, 02:01 | 48 | 0.8-7.6 | 0.16-1.52 |
| COV318 | Ovarian carcinoma | + | 03:01, 02:01 | 48 | 4 | 0.8 |
| Ovcar-3 | Ovarian carcinoma | + | 02:01, 29:02 | 48 | 10.8 | 2.16 |
| T98G | Glioblastoma | + | 02:01, 02:01 | 48 | 0.5 | 0.1 |
| MDA-MB-231 | Breast adenocarcinoma | + | 02:01, 02:17 | 48 | 0.87 | 0.174 |
| BV173 | Blast phase chronic myelogenous leukemia | + | 30:01, 02:01 | 48 | 0.97-13.75 | 0.19-2.75 |
| SW480 | Colorectal adenocarcinoma | + | 02:01, 24:02 | 48 | 0.05-5.4 | 0.01-1.08 |
| SW620 | Colorectal adenocarcinoma | + | 02:01, 24:02 | 48 | 5.8-8.5 | 1.16-1.7 |
| HEC-50B | Endometrial adenocarcinoma | + | 02:07, 02:07 | 48 | 5.95-6.2 | 1.19-1.24 |
| ARH-77 | Plasma cell leukemia | — | 02:01, 03:01 | NA | NA | NA |
| HL-60 | Promyelocytic leukemia | + | 01:01 | NA | NA | NA |
| Cell line . | Disease of tissue origin . | WT1 mRNA . | HLA-A typing based on TRON database . | Time of EC50 evaluation (h) . | EC50 (nM) T-cell killing in vitro . | EC50 (µg/mL) T-cell killing in vitro . |
|---|---|---|---|---|---|---|
| SKM-1 | AML | + | 33:03, 02:07 | 44 | 0.16-1.25 | 0.032-0.25 |
| OCI-M1 | AML | + | 03:01, 02:01 | 68 | 0.75-2.15 | 0.15-0.43 |
| OCI-AML3 | AML | + | 23:01, 02:01 | 68 | 0.20-6.75 | 0.04-1.35 |
| Set-2 | AML | + | 33:03, 02:01 | 48 | 0.8-7.6 | 0.16-1.52 |
| COV318 | Ovarian carcinoma | + | 03:01, 02:01 | 48 | 4 | 0.8 |
| Ovcar-3 | Ovarian carcinoma | + | 02:01, 29:02 | 48 | 10.8 | 2.16 |
| T98G | Glioblastoma | + | 02:01, 02:01 | 48 | 0.5 | 0.1 |
| MDA-MB-231 | Breast adenocarcinoma | + | 02:01, 02:17 | 48 | 0.87 | 0.174 |
| BV173 | Blast phase chronic myelogenous leukemia | + | 30:01, 02:01 | 48 | 0.97-13.75 | 0.19-2.75 |
| SW480 | Colorectal adenocarcinoma | + | 02:01, 24:02 | 48 | 0.05-5.4 | 0.01-1.08 |
| SW620 | Colorectal adenocarcinoma | + | 02:01, 24:02 | 48 | 5.8-8.5 | 1.16-1.7 |
| HEC-50B | Endometrial adenocarcinoma | + | 02:07, 02:07 | 48 | 5.95-6.2 | 1.19-1.24 |
| ARH-77 | Plasma cell leukemia | — | 02:01, 03:01 | NA | NA | NA |
| HL-60 | Promyelocytic leukemia | + | 01:01 | NA | NA | NA |
NA; not applicable.