Table 2. Formulations for Ussing chamber... | American Society of Hematology

Table 2.

Formulations for Ussing chamber flux and in vivo ⁵⁹Fe absorption studies*

Compound	Concentration (mM)	AA†	Concentration (mM)
NaCl	103 (86)‡	Aspartic acid	8
KCl	5.2	Glutamine	8
NaH₂PO₄	7.6	Glycine	8
Na₂HPO₄	1.4	Glutamic acid§	6.3
MgCl₂	1.2
CaCl₂· 2H₂O	1.2
Na citrate monobasic	25
FeSO₄ · 7 H₂O	1.5ǁ

Compound	Concentration (mM)	AA†	Concentration (mM)
NaCl	103 (86)‡	Aspartic acid	8
KCl	5.2	Glutamine	8
NaH₂PO₄	7.6	Glycine	8
Na₂HPO₄	1.4	Glutamic acid§	6.3
MgCl₂	1.2
CaCl₂· 2H₂O	1.2
Na citrate monobasic	25
FeSO₄ · 7 H₂O	1.5ǁ

Adjusted to pH 6.5; osmolarity = 296 mOsm (control formulation), 294 mOsm (4 AA formulation).

No AAs were added to the control formulation.

103 mM (control formulation), 86 mM (4 AA formulation).

Monosodium, monohydrate.

Omitted for in vivo iron absorption studies (unless stated otherwise).