Novel mHA characteristics
| mHA name . | mHA . | HLA allele . | Gene . | Chromosome . | rsID of SNP . | Donor amino acid . | Recipient amino acid . | Major allele . | Minor allele . | MAF in TOPMED . | MAF in ALFA . | MAF in 1000 genomes . | Peptide length . | Binding affinity . | Frequency in DISCOVeRY-BMT patients with corresponding HLA . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UNC-IQCE-V | AVLDEAVV | A∗02:01 | IQCE | 11 | rs2293404 | A | V | C | T | 0.35 | 0.29 | 0.41 | 8 | 412.7421 | 26.4 |
| UNC-GLRX3-S | FLSSANEHL | A∗02:01 | GLRX3 | 10 | rs2274217 | P | S | C | T | 0.21 | 0.25 | 0.19 | 9 | 14.9364 | 25.6 |
| UNC-SLC25A37-V | AQYTSVYGA | A∗02:01 | SLC25A37 | 8 | rs2942194 | I | V | A | G | 0.21 | 0.26 | 0.17 | 9 | 45.2204 | 25.3 |
| UNC-ARHGEF18-Q | SLICRQLGSA | A∗02:01 | ARHGEF18 | 19 | rs2287918 | R | Q | G | A | 0.19 | 0.24 | 0.17 | 10 | 367.3796 | 23.9 |
| UNC-DPP3-H | KLIVQPNTHL | A∗02:01 | DPP3 | 11 | rs2305535 | R | H | G | A | 0.19 | 0.22 | 0.21 | 10 | 218.2962 | 23.6 |
| UNC-HEXDC-V | RLHVGCDEV | A∗02:01 | HEXDC | 17 | rs4789773 | I | V | A | G | 0.45 | 0.37 | 0.56 | 9 | 357.9789 | 24 |
| UNC-TOP1MT-W | WLLEKLQEQL | A∗02:01 | TOP1MT | 8 | rs2293925 | R | W | C | T | 0.36 | 0.43 | 0.46 | 10 | 8.8962 | 21.1 |
| UNC-USP4-V | KVSFFVPRL | A∗02:01 | USP4 | 3 | rs35446411 | L | V | T | G | 0.13 | 0.16 | 0.09 | 9 | 445.1234 | 22.4 |
| UNC-AHRR-A | VVFGQAPPL | A∗02:01 | AHRR | 5 | rs2292596 | P | A | C | G | 0.30 | 0.35 | 0.38 | 9 | 311.398 | 21.7 |
| UNC-FPR1-K | KVAVAMLTV | A∗02:01 | FPR1 | 19 | rs1042229 | N | K | T | G | — | 0.45 | 0.37 | 9 | 178.6526 | 28.3 |
| UNC-FLT3-G | ALARGGGQLPL | A∗02:01 | FLT3 | 13 | rs12872889 | D | G | A | G | 0.35 | 0.31 | 0.37 | 11 | 257.8616 | 24.3 |
| UNC-GDPD5-A | ALSQVPSPL | A∗02:01 | GDPD5 | 11 | rs571353 | T | A | A | G | 0.34 | 0.28 | 0.33 | 9 | 94.9686 | 23.6 |
| UNC-SLC26A8-M | FLRCMLTI | A∗02:01 | SLC26A8 | 6 | rs743923 | V | M | G | A | 0.30 | 0.25 | 0.26 | 8 | 100.0366 | 25 |
| UNC-FPGS-I | FLAAASARGI | A∗02:01 | FPGS | 9 | rs10760502 | V | I | C | T | 0.28 | 0.35 | 0.22 | 10 | 23.9163 | 26.5 |
| UNC-NDUFAF1-L | ALYPFLGIL | A∗02:01 | NDUFAF1 | 15 | rs3204853 | R | L | C | A | 0.17 | 0.24 | 0.12 | 9 | 164.2741 | 26.4 |
| UNC-WDR62-L | LLGDDDVADGL | A∗02:01 | WDR62 | 19 | rs2285745 | S | L | C | T | 0.32 | 0.35 | 0.35 | 11 | 182.9111 | 26.1 |
| UNC-POLL-W | HPDGWSHRGIF | B∗35:01 | POLL | 10 | rs3730477 | R | W | C | T | 0.16 | 0.21 | 0.10 | 11 | 31.3362 | 27.4 |
| UNC-HLX-P | LPAAYHHH | B∗35:01 | HLX | 1 | rs12141189 | S | P | T | C | 0.23 | 0.23 | 0.21 | 8 | 285.0737 | 26.6 |
| UNC-NEK4-A | LPAMPRDY | B∗35:01 | NEK4 | 3 | rs1029871 | P | A | G | C | 0.33 | 0.39 | 0.31 | 8 | 39.4681 | 26 |
| UNC-MARCH2-T | GRLLSTVIRTL | C∗07:02 | MARCH2 | 19 | rs1133893 | A | T | C | T | 0.24 | 0.32 | 0.20 | 11 | 175.8924 | 26.7 |
| UNC-GAA-R | RRQLDGRVLL | C∗07:02 | GAA | 17 | rs1042395 | H | R | A | G | 0.36 | 0.28 | 0.40 | 10 | 375.2756 | 25.4 |
| UNC-RNASE3-R | RYADRPGRRF | C∗07:02 | RNASE3 | 14 | rs2073342 | T | R | C | G | 0.36 | 0.29 | 0.36 | 10 | 147.8419 | 25.9 |
| UNC-SNX19-V | FLQPNVRGPLF | C∗07:02 | SNX19 | 11 | rs3751037 | L | V | G | C | 0.30 | 0.29 | 0.27 | 11 | 161.7018 | 25.8 |
| UNC-BCL2A1-Y | YRLAQDYLQY | C∗07:02 | BCL2A1 | 15 | rs1138357 | C | Y | G | A | 0.28 | 0.26 | 0.35 | 10 | 188.9083 | 24.4 |
| mHA name . | mHA . | HLA allele . | Gene . | Chromosome . | rsID of SNP . | Donor amino acid . | Recipient amino acid . | Major allele . | Minor allele . | MAF in TOPMED . | MAF in ALFA . | MAF in 1000 genomes . | Peptide length . | Binding affinity . | Frequency in DISCOVeRY-BMT patients with corresponding HLA . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UNC-IQCE-V | AVLDEAVV | A∗02:01 | IQCE | 11 | rs2293404 | A | V | C | T | 0.35 | 0.29 | 0.41 | 8 | 412.7421 | 26.4 |
| UNC-GLRX3-S | FLSSANEHL | A∗02:01 | GLRX3 | 10 | rs2274217 | P | S | C | T | 0.21 | 0.25 | 0.19 | 9 | 14.9364 | 25.6 |
| UNC-SLC25A37-V | AQYTSVYGA | A∗02:01 | SLC25A37 | 8 | rs2942194 | I | V | A | G | 0.21 | 0.26 | 0.17 | 9 | 45.2204 | 25.3 |
| UNC-ARHGEF18-Q | SLICRQLGSA | A∗02:01 | ARHGEF18 | 19 | rs2287918 | R | Q | G | A | 0.19 | 0.24 | 0.17 | 10 | 367.3796 | 23.9 |
| UNC-DPP3-H | KLIVQPNTHL | A∗02:01 | DPP3 | 11 | rs2305535 | R | H | G | A | 0.19 | 0.22 | 0.21 | 10 | 218.2962 | 23.6 |
| UNC-HEXDC-V | RLHVGCDEV | A∗02:01 | HEXDC | 17 | rs4789773 | I | V | A | G | 0.45 | 0.37 | 0.56 | 9 | 357.9789 | 24 |
| UNC-TOP1MT-W | WLLEKLQEQL | A∗02:01 | TOP1MT | 8 | rs2293925 | R | W | C | T | 0.36 | 0.43 | 0.46 | 10 | 8.8962 | 21.1 |
| UNC-USP4-V | KVSFFVPRL | A∗02:01 | USP4 | 3 | rs35446411 | L | V | T | G | 0.13 | 0.16 | 0.09 | 9 | 445.1234 | 22.4 |
| UNC-AHRR-A | VVFGQAPPL | A∗02:01 | AHRR | 5 | rs2292596 | P | A | C | G | 0.30 | 0.35 | 0.38 | 9 | 311.398 | 21.7 |
| UNC-FPR1-K | KVAVAMLTV | A∗02:01 | FPR1 | 19 | rs1042229 | N | K | T | G | — | 0.45 | 0.37 | 9 | 178.6526 | 28.3 |
| UNC-FLT3-G | ALARGGGQLPL | A∗02:01 | FLT3 | 13 | rs12872889 | D | G | A | G | 0.35 | 0.31 | 0.37 | 11 | 257.8616 | 24.3 |
| UNC-GDPD5-A | ALSQVPSPL | A∗02:01 | GDPD5 | 11 | rs571353 | T | A | A | G | 0.34 | 0.28 | 0.33 | 9 | 94.9686 | 23.6 |
| UNC-SLC26A8-M | FLRCMLTI | A∗02:01 | SLC26A8 | 6 | rs743923 | V | M | G | A | 0.30 | 0.25 | 0.26 | 8 | 100.0366 | 25 |
| UNC-FPGS-I | FLAAASARGI | A∗02:01 | FPGS | 9 | rs10760502 | V | I | C | T | 0.28 | 0.35 | 0.22 | 10 | 23.9163 | 26.5 |
| UNC-NDUFAF1-L | ALYPFLGIL | A∗02:01 | NDUFAF1 | 15 | rs3204853 | R | L | C | A | 0.17 | 0.24 | 0.12 | 9 | 164.2741 | 26.4 |
| UNC-WDR62-L | LLGDDDVADGL | A∗02:01 | WDR62 | 19 | rs2285745 | S | L | C | T | 0.32 | 0.35 | 0.35 | 11 | 182.9111 | 26.1 |
| UNC-POLL-W | HPDGWSHRGIF | B∗35:01 | POLL | 10 | rs3730477 | R | W | C | T | 0.16 | 0.21 | 0.10 | 11 | 31.3362 | 27.4 |
| UNC-HLX-P | LPAAYHHH | B∗35:01 | HLX | 1 | rs12141189 | S | P | T | C | 0.23 | 0.23 | 0.21 | 8 | 285.0737 | 26.6 |
| UNC-NEK4-A | LPAMPRDY | B∗35:01 | NEK4 | 3 | rs1029871 | P | A | G | C | 0.33 | 0.39 | 0.31 | 8 | 39.4681 | 26 |
| UNC-MARCH2-T | GRLLSTVIRTL | C∗07:02 | MARCH2 | 19 | rs1133893 | A | T | C | T | 0.24 | 0.32 | 0.20 | 11 | 175.8924 | 26.7 |
| UNC-GAA-R | RRQLDGRVLL | C∗07:02 | GAA | 17 | rs1042395 | H | R | A | G | 0.36 | 0.28 | 0.40 | 10 | 375.2756 | 25.4 |
| UNC-RNASE3-R | RYADRPGRRF | C∗07:02 | RNASE3 | 14 | rs2073342 | T | R | C | G | 0.36 | 0.29 | 0.36 | 10 | 147.8419 | 25.9 |
| UNC-SNX19-V | FLQPNVRGPLF | C∗07:02 | SNX19 | 11 | rs3751037 | L | V | G | C | 0.30 | 0.29 | 0.27 | 11 | 161.7018 | 25.8 |
| UNC-BCL2A1-Y | YRLAQDYLQY | C∗07:02 | BCL2A1 | 15 | rs1138357 | C | Y | G | A | 0.28 | 0.26 | 0.35 | 10 | 188.9083 | 24.4 |
16 novel GVL mHAs that bind HLA-A∗02:01, 3 that bind B∗35:01, and 5 that bind C∗07:02 and were validated by mass spectrometry are shown.
MAF, minor allele frequency.