Kinetic inhibition of native ADAMTS13 in plasma with various antispacer domain inhibitors
| Inhibitor . | Concentration (nM) . | Vmax (FU/s) . | K0.5 (μM) . | αapp (global-fit) . |
|---|---|---|---|---|
| scFv4-20 | 0 | 2.79 ± 0.07 | 1.71 ± 0.05 | 1.20 ± 0.10 |
| 0.04 | 2.72 ± 0.08 | 1.69 ± 0.11 | ||
| 0.12 | 2.45 ± 0.13 | 1.75 ± 0.19 | ||
| 0.37 | 1.97 ± 0.14 | 1.94 ± 0.33 | ||
| 1.1 | 1.10 ± 0.09 | 1.60 ± 0.12 | ||
| scFv3-1 | 0 | 2.19 ± 0.12 | 0.58 ± 0.07 | 2.78 ± 0.26 |
| 1.1 | 2.18 ± 0.11 | 0.77 ± 0.13 | ||
| 3.3 | 1.97 ± 0.04 | 0.87 ± 0.08 | ||
| 10 | 1.56 ± 0.10 | 1.12 ± 0.21 | ||
| 30 | 1.56 ± 0.28 | 2.46 ± 0.52 | ||
| scFv4-16 | 0 | 2.01 ± 0.06 | 0.45 ± 0.02 | 1.61 ± 0.13 |
| 0.37 | 1.95 ± 0.03 | 0.46 ± 0.03 | ||
| 1.1 | 1.91 ± 0.06 | 0.49 ± 0.04 | ||
| 3.3 | 1.64 ±0.03 | 0.53 ± 0.03 | ||
| 10 | 1.11 ± 0.08 | 0.63 ± 0.08 |
| Inhibitor . | Concentration (nM) . | Vmax (FU/s) . | K0.5 (μM) . | αapp (global-fit) . |
|---|---|---|---|---|
| scFv4-20 | 0 | 2.79 ± 0.07 | 1.71 ± 0.05 | 1.20 ± 0.10 |
| 0.04 | 2.72 ± 0.08 | 1.69 ± 0.11 | ||
| 0.12 | 2.45 ± 0.13 | 1.75 ± 0.19 | ||
| 0.37 | 1.97 ± 0.14 | 1.94 ± 0.33 | ||
| 1.1 | 1.10 ± 0.09 | 1.60 ± 0.12 | ||
| scFv3-1 | 0 | 2.19 ± 0.12 | 0.58 ± 0.07 | 2.78 ± 0.26 |
| 1.1 | 2.18 ± 0.11 | 0.77 ± 0.13 | ||
| 3.3 | 1.97 ± 0.04 | 0.87 ± 0.08 | ||
| 10 | 1.56 ± 0.10 | 1.12 ± 0.21 | ||
| 30 | 1.56 ± 0.28 | 2.46 ± 0.52 | ||
| scFv4-16 | 0 | 2.01 ± 0.06 | 0.45 ± 0.02 | 1.61 ± 0.13 |
| 0.37 | 1.95 ± 0.03 | 0.46 ± 0.03 | ||
| 1.1 | 1.91 ± 0.06 | 0.49 ± 0.04 | ||
| 3.3 | 1.64 ±0.03 | 0.53 ± 0.03 | ||
| 10 | 1.11 ± 0.08 | 0.63 ± 0.08 |
Each inhibitor binds to the spacer domain of ADAMTS13. Reaction conditions: temperature 25°C, pH 6.0. All data are presented as mean ± SEM.