Kinetic inhibition of plasma ADAMTS13 at 37°C and pH of 6.0
| Inhibitor . | Concentration (nM) . | Vmax (FU/s) . | K0.5 (μM) . | αapp (global-fit) . |
|---|---|---|---|---|
| scFv4-20 | 0 | 2.73 ± 0.12 | 0.92 ± 0.08 | 1.71 ± 0.07 |
| 0.37 | 2.80 ± 0.12 | 1.00 ± 0.06 | ||
| 1.1 | 2.49 ± 0.12 | 0.93 ± 0.11 | ||
| 3.3 | 2.41 ± 0.10 | 1.23 ± 0.11 | ||
| 10 | 1.64 ± 0.12 | 1.28 ± 0.08 | ||
| scFv3-1 | 0 | 2.21 ± 0.21 | 1.29 ± 0.29 | 1.17 ± 0.15 |
| 1.1 | 2.94 ± 0.85 | 2.49 ± 1.26 | ||
| 3.3 | 2.22 ± 0.38 | 1.74 ± 0.50 | ||
| 10 | 1.80 ± 0.40 | 1.65 ± 0.55 | ||
| 30 | 1.50 ± 0.33 | 2.39 ± 0.81 | ||
| scFv4-16 | 0 | 2.11 ± 0.15 | 1.28 ± 0.14 | 1.06 ± 0.06 |
| 1.1 | 1.93 ± 0.08 | 1.21 ± 0.10 | ||
| 3.3 | 1.70 ± 0.10 | 1.28 ± 0.10 | ||
| 10 | 1.26 ± 0.09 | 1.47 ± 0.25 | ||
| 30 | 1.76 ± 0.11 | 1.44 ± 0.26 |
| Inhibitor . | Concentration (nM) . | Vmax (FU/s) . | K0.5 (μM) . | αapp (global-fit) . |
|---|---|---|---|---|
| scFv4-20 | 0 | 2.73 ± 0.12 | 0.92 ± 0.08 | 1.71 ± 0.07 |
| 0.37 | 2.80 ± 0.12 | 1.00 ± 0.06 | ||
| 1.1 | 2.49 ± 0.12 | 0.93 ± 0.11 | ||
| 3.3 | 2.41 ± 0.10 | 1.23 ± 0.11 | ||
| 10 | 1.64 ± 0.12 | 1.28 ± 0.08 | ||
| scFv3-1 | 0 | 2.21 ± 0.21 | 1.29 ± 0.29 | 1.17 ± 0.15 |
| 1.1 | 2.94 ± 0.85 | 2.49 ± 1.26 | ||
| 3.3 | 2.22 ± 0.38 | 1.74 ± 0.50 | ||
| 10 | 1.80 ± 0.40 | 1.65 ± 0.55 | ||
| 30 | 1.50 ± 0.33 | 2.39 ± 0.81 | ||
| scFv4-16 | 0 | 2.11 ± 0.15 | 1.28 ± 0.14 | 1.06 ± 0.06 |
| 1.1 | 1.93 ± 0.08 | 1.21 ± 0.10 | ||
| 3.3 | 1.70 ± 0.10 | 1.28 ± 0.10 | ||
| 10 | 1.26 ± 0.09 | 1.47 ± 0.25 | ||
| 30 | 1.76 ± 0.11 | 1.44 ± 0.26 |
Each inhibitor binds to the spacer domain of ADAMTS13. All data are presented as mean ± SEM.