Kinetic inhibition of plasma ADAMTS13 at 25°C and pH of 6.31
| Inhibitor . | Concentration (nM) . | Vmax (FU/s) . | K0.5 (μM) . | αapp (global-fit) . |
|---|---|---|---|---|
| scFv4-20 | 0 | 2.96 ± 0.05 | 1.48 ± 0.03 | 1.09 ± 0.17 |
| 0.04 | 2.83 ± 0.15 | 1.44 ± 0.06 | ||
| 0.12 | 2.36 ± 0.22 | 1.46 ± 0.23 | ||
| 0.37 | 1.87 ± 0.05 | 1.66 ± 0.13 | ||
| 1.1 | 1.14 ± 0.18 | 1.61 ± 0.20 | ||
| scFv3-1 | 0 | 2.88 ± 0.15 | 1.35 ± 0.08 | 1.42 ± 0.22 |
| 1.1 | 2.71 ± 0.22 | 1.43 ± 0.14 | ||
| 3.3 | 2.51 ± 0.24 | 1.56 ± 0.19 | ||
| 10 | 2.03 ± 0.07 | 1.69 ± 0.21 | ||
| 30 | 1.72 ± 0.07 | 2.36 ± 0.32 | ||
| scFv4-16 | 0 | 2.67 ± 0.20 | 1.36 ± 0.11 | 1.27 ± 0.19 |
| 1.1 | 2.32 ± 0.18 | 1.38 ± 0.06 | ||
| 3.3 | 2.06 ± 0.21 | 1.45 ± 0.10 | ||
| 10 | 1.45 ± 0.13 | 1.69 ± 0.17 | ||
| 30 | 1.00 ± 0.29 | 2.45 ± 1.04 |
| Inhibitor . | Concentration (nM) . | Vmax (FU/s) . | K0.5 (μM) . | αapp (global-fit) . |
|---|---|---|---|---|
| scFv4-20 | 0 | 2.96 ± 0.05 | 1.48 ± 0.03 | 1.09 ± 0.17 |
| 0.04 | 2.83 ± 0.15 | 1.44 ± 0.06 | ||
| 0.12 | 2.36 ± 0.22 | 1.46 ± 0.23 | ||
| 0.37 | 1.87 ± 0.05 | 1.66 ± 0.13 | ||
| 1.1 | 1.14 ± 0.18 | 1.61 ± 0.20 | ||
| scFv3-1 | 0 | 2.88 ± 0.15 | 1.35 ± 0.08 | 1.42 ± 0.22 |
| 1.1 | 2.71 ± 0.22 | 1.43 ± 0.14 | ||
| 3.3 | 2.51 ± 0.24 | 1.56 ± 0.19 | ||
| 10 | 2.03 ± 0.07 | 1.69 ± 0.21 | ||
| 30 | 1.72 ± 0.07 | 2.36 ± 0.32 | ||
| scFv4-16 | 0 | 2.67 ± 0.20 | 1.36 ± 0.11 | 1.27 ± 0.19 |
| 1.1 | 2.32 ± 0.18 | 1.38 ± 0.06 | ||
| 3.3 | 2.06 ± 0.21 | 1.45 ± 0.10 | ||
| 10 | 1.45 ± 0.13 | 1.69 ± 0.17 | ||
| 30 | 1.00 ± 0.29 | 2.45 ± 1.04 |
Each inhibitor binds to the spacer domain of ADAMTS13. All data are presented as mean ± SEM.