Kinetic parameters of the prothrombinase activity of FV mutants
| . | Kd . | Km . | |
|---|---|---|---|
| FV mutant | PL | FXa | Prothrombin |
| μM | nM | μM | |
| FV-WT | 1.02 ± 0.21 | 0.061 ± 0.016 | 0.675 ± 0.126 |
| FV-A2086D | 22.2 ± 2.8 | 0.129 ± 0.013 | 0.708 ± 0.066 |
| . | Kd . | Km . | |
|---|---|---|---|
| FV mutant | PL | FXa | Prothrombin |
| μM | nM | μM | |
| FV-WT | 1.02 ± 0.21 | 0.061 ± 0.016 | 0.675 ± 0.126 |
| FV-A2086D | 22.2 ± 2.8 | 0.129 ± 0.013 | 0.708 ± 0.066 |
Reactions performed are described in “Materials and methods.” Kinetic parameters were calculated by fitting the data presented in Figure 3. Data are shown as average ± standard deviation values.