Binding parameters of the interaction of FV mutants and immobilized EGR-APC or PS in an SPR-based assay (BIAcore)
| . | EGR-APC immobilized . | PS immobilized . | ||||||
|---|---|---|---|---|---|---|---|---|
| FV mutant | kass | kdiss | Kd | Chi2 | kass | kdiss | Kd | χ2 |
| ×105 M−1s−1 | ×10−3 s−1 | nM | ×105 M−1s−1 | ×10−3 s−1 | nM | |||
| WT | 3.37 | 3.59 | 10.7 | 1.29 | 5.20 | 3.93 | 7.56 | 1.76 |
| A2086D | 1.32 | 2.68 | 20.3 | 0.824 | 9.84 | 1.93 | 19.6 | 0.919 |
| W1920R | 3.38 | 4.33 | 12.8 | 0.832 | 1.45 | 3.82 | 26.2 | 0.708 |
| R506Q | 2.39 | 4.57 | 19.1 | 0.106 | 7.11 | 3.74 | 5.26 | 0.960 |
| . | EGR-APC immobilized . | PS immobilized . | ||||||
|---|---|---|---|---|---|---|---|---|
| FV mutant | kass | kdiss | Kd | Chi2 | kass | kdiss | Kd | χ2 |
| ×105 M−1s−1 | ×10−3 s−1 | nM | ×105 M−1s−1 | ×10−3 s−1 | nM | |||
| WT | 3.37 | 3.59 | 10.7 | 1.29 | 5.20 | 3.93 | 7.56 | 1.76 |
| A2086D | 1.32 | 2.68 | 20.3 | 0.824 | 9.84 | 1.93 | 19.6 | 0.919 |
| W1920R | 3.38 | 4.33 | 12.8 | 0.832 | 1.45 | 3.82 | 26.2 | 0.708 |
| R506Q | 2.39 | 4.57 | 19.1 | 0.106 | 7.11 | 3.74 | 5.26 | 0.960 |
Reactions were performed are described in “Materials and methods.” Parameter values were calculated by nonlinear regression analysis from data in Figure 4A-B using the evaluation software provided by Biacore AB.
EGR-APC, EGR active site-blocked APC.