Antibodies used in multiplexed immunohistochemistry
| Position . | Antibody . | Clone/host . | Company/item . | Concentration μg/mL . | OPAL fluor . |
|---|---|---|---|---|---|
| 1 | CD3 | SP7 / rabbit | Thermo Fisher Scientific / RM-9107 | 0.06 (1:400) | 520 |
| 2 | CD19 | UMAB103 / mouse | Origene / UM500071 | 0.5 (1:2000) | 690 |
| 3 | CD33 | RBT-CD33 / rabbit | Bio SB Inc / BSB3451 | 1 (1:100) | 570 |
| 4 | CD79A | JCB117 / mouse | Bio SB Inc / BSB5305 | 0.166 (1:600) | 540 |
| 5 | CD79B | EPR6861 / rabbit | Abcam / ab134147 | 0.065 (1:1600) | 620 |
| 6 | Cyclin-D | SP4 / rabbit | Sigma / SAB5500090 | 0.625 (1:400) | 650 |
| Secondary | Opal polymer HRP Ms+Rb | Akoya Biosciences / ARH1001EA | Ready to use | ||
| Position . | Antibody . | Clone/host . | Company/item . | Concentration μg/mL . | OPAL fluor . |
|---|---|---|---|---|---|
| 1 | CD3 | SP7 / rabbit | Thermo Fisher Scientific / RM-9107 | 0.06 (1:400) | 520 |
| 2 | CD19 | UMAB103 / mouse | Origene / UM500071 | 0.5 (1:2000) | 690 |
| 3 | CD33 | RBT-CD33 / rabbit | Bio SB Inc / BSB3451 | 1 (1:100) | 570 |
| 4 | CD79A | JCB117 / mouse | Bio SB Inc / BSB5305 | 0.166 (1:600) | 540 |
| 5 | CD79B | EPR6861 / rabbit | Abcam / ab134147 | 0.065 (1:1600) | 620 |
| 6 | Cyclin-D | SP4 / rabbit | Sigma / SAB5500090 | 0.625 (1:400) | 650 |
| Secondary | Opal polymer HRP Ms+Rb | Akoya Biosciences / ARH1001EA | Ready to use | ||