Summary of results with WT and mutant STAT3 proteins
| STAT3 protein . | WT . | R382W . | F384S . | H437P . | V463del . | R423Q . | Q344H . | S636Y . | S668Y . | T6221 . | Y657S . | V637M . | N647D . | Y640F . | K658Y . | D661Y . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Domain | NA | DBD | SH2D | |||||||||||||
| Classification—LOF vs GOF | NA | LOF | GOF | LOF | GOF | |||||||||||
| Classification—F, S, or S/F | NA | F | S | S | S | S/F | NA | F | F | S | S | S/F | S/F | NA | NA | NA |
| pY-STAT3—w/o IL6/sIL6R | - | - | - | - | - | - | + | - | - | - | - | - | - | + | + | + |
| pY-STAT3—with IL6/sIL6R | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + | ++ | ++ | ++ | ++ | +++ | +++ | +++ | +++ |
| pY-STAT3—with v-Src | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + | + | + | + | + | + | +++ | +++ | +++ |
| pY1068—pull-down | +++ | +++ | +++ | +++ | +++ | +++ | +++ | - | ++ | - | + | + | ++ | + | + | +++ |
| pY1068—SPR | +++ | + | ND | ND | ++ | ++ | + | - | ++ | - | - | + | + | + | + | + |
| Homodimerization | +++ | +++ | +++ | +++ | +++ | +++ | +++ | - | + | + | + | + | +++ | ++ | ++ | ++ |
| Nuclear accumulation | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | + | + | + | ++ | ++ | ++ | ++ |
| DNA binding—SPR | +++ | - | ND | ND | - | - | ++++ | ++ | ++ | + | - | ++ | ++ | +++ | ++++ | ++++ |
| DNA binding—ELISA | ++ | - | - | - | - | + | ++ | - | - | - | - | - | - | ++++ | ++++ | ++++ |
| STAT3 protein . | WT . | R382W . | F384S . | H437P . | V463del . | R423Q . | Q344H . | S636Y . | S668Y . | T6221 . | Y657S . | V637M . | N647D . | Y640F . | K658Y . | D661Y . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Domain | NA | DBD | SH2D | |||||||||||||
| Classification—LOF vs GOF | NA | LOF | GOF | LOF | GOF | |||||||||||
| Classification—F, S, or S/F | NA | F | S | S | S | S/F | NA | F | F | S | S | S/F | S/F | NA | NA | NA |
| pY-STAT3—w/o IL6/sIL6R | - | - | - | - | - | - | + | - | - | - | - | - | - | + | + | + |
| pY-STAT3—with IL6/sIL6R | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + | ++ | ++ | ++ | ++ | +++ | +++ | +++ | +++ |
| pY-STAT3—with v-Src | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + | + | + | + | + | + | +++ | +++ | +++ |
| pY1068—pull-down | +++ | +++ | +++ | +++ | +++ | +++ | +++ | - | ++ | - | + | + | ++ | + | + | +++ |
| pY1068—SPR | +++ | + | ND | ND | ++ | ++ | + | - | ++ | - | - | + | + | + | + | + |
| Homodimerization | +++ | +++ | +++ | +++ | +++ | +++ | +++ | - | + | + | + | + | +++ | ++ | ++ | ++ |
| Nuclear accumulation | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | + | + | + | ++ | ++ | ++ | ++ |
| DNA binding—SPR | +++ | - | ND | ND | - | - | ++++ | ++ | ++ | + | - | ++ | ++ | +++ | ++++ | ++++ |
| DNA binding—ELISA | ++ | - | - | - | - | + | ++ | - | - | - | - | - | - | ++++ | ++++ | ++++ |
F, functional; NA, not applicable; ND, not done; S, structural; S/F, structural-functional.22