Homolog peptides identified in human exome by in silico screening
| Sequences tested for cross-reactivity to RMFPNAPYL∗ . | Epitope name . | Sequences found in exome (<1 μM Kd) . | Source protein . | HLA-A2 binding (KD prediction) . |
|---|---|---|---|---|
| X(LMV)FPNAPY(LVI) | WT1-RMF | RMFPNAPYL | WT1 | 7 nM |
| X(LMV)FPNAPY(LVI) And substitute any other position with any conserved amino acid | ABHD3-9-mer | SLYPSAPFL | Phospholipase ABHD3 | 7 nM |
| Same as previous row but with 10 residues instead | ABHD3-10-mer | SLYPSAPFLA | Phospholipase ABHD3 | 14 nM |
| AMFR | EMFPQVPYHL | Autocrine motility factor receptor | 148 nM | |
| Identified previously (Ref 20) | TSTP1-10-mer | RLFPNAKFLL | Tyrosyl protein sulfotransferase 1 | 27 nM |
| Sequences tested for cross-reactivity to RMFPNAPYL∗ . | Epitope name . | Sequences found in exome (<1 μM Kd) . | Source protein . | HLA-A2 binding (KD prediction) . |
|---|---|---|---|---|
| X(LMV)FPNAPY(LVI) | WT1-RMF | RMFPNAPYL | WT1 | 7 nM |
| X(LMV)FPNAPY(LVI) And substitute any other position with any conserved amino acid | ABHD3-9-mer | SLYPSAPFL | Phospholipase ABHD3 | 7 nM |
| Same as previous row but with 10 residues instead | ABHD3-10-mer | SLYPSAPFLA | Phospholipase ABHD3 | 14 nM |
| AMFR | EMFPQVPYHL | Autocrine motility factor receptor | 148 nM | |
| Identified previously (Ref 20) | TSTP1-10-mer | RLFPNAKFLL | Tyrosyl protein sulfotransferase 1 | 27 nM |
Potential off-target sequences were identified using ScanProsite (Expasy) to search the human exome (UniProtKB/Swiss-Prot) for homologous 9 amino acid peptides that had predicted affinity to HLA-A∗02:01 (HLA-A2) of <500 nM using NetMHCPan.
The rules used to identify potential off-target sequences are as follows: position no. 1 could be any amino acid “X”; position no. 2 and no. 9 could be any canonical anchor (LMV) and (LVI). Position no.3 to no.8 could be replaced with any conserved residues, shown hereafter, at their respective positions in RMFPNAPYL: F→Y = W; P→P; N→S = T = Q; A→V = I = G; Y→F = W. Additional homologous 10-mers were also included. TSTP1 was identified in a previous study.20