Table 4. Reduction of primary AML by... | American Society of Hematology

Table 4.

Reduction of primary AML by AbTCR and AbTCR-CSR T cells

Primary AML	Reduction of CD33⁺ cells (%)					HLA-A∗02:01	WT1 RMF/HLA-A2 complex expression	CD33 expression
Primary AML	CSR only	AbTCR no. 18 only	AbTCR no. 34 only	AbTCR-CSR no. 18	AbTCR-CSR no. 34	HLA-A∗02:01	WT1 RMF/HLA-A2 complex expression	CD33 expression
60D	None	73	28	90	16	+	+	99%
114B	None	35	38	81	17	+	+	54%
92C	None	57	69	69	74	+	+	77%
120B	None	5.6	None	None	2.3	−	−	40%
AML-14	None	41	55	74	55	+	+	99%

Primary AML	Reduction of CD33⁺ cells (%)					HLA-A∗02:01	WT1 RMF/HLA-A2 complex expression	CD33 expression
Primary AML	CSR only	AbTCR no. 18 only	AbTCR no. 34 only	AbTCR-CSR no. 18	AbTCR-CSR no. 34	HLA-A∗02:01	WT1 RMF/HLA-A2 complex expression	CD33 expression
60D	None	73	28	90	16	+	+	99%
114B	None	35	38	81	17	+	+	54%
92C	None	57	69	69	74	+	+	77%
120B	None	5.6	None	None	2.3	−	−	40%
AML-14	None	41	55	74	55	+	+	99%

Primary AML samples were labeled with either CFSE or far-red and coincubated with ESK2 AbTCR or AbTCR-CSR T cells at an effector-to-target ratio of 1:1, overnight. The cells were harvested and were stained with anti-CD33 mAb and analyzed by flow cytometry. The cells were gated on live cells and the same gates were applied to all groups. The percentage of CD33 and CFSE-positive (or far-red–positive) cells in the live cell gates were analyzed (as shown in supplemental Figure 6), and the percentage of total CD33⁺CFSE/far-red⁺ cells were calculated (including CD33^low cells in AbTCR-CSR groups).

Percentage reduction of the AML cells by AbTCR, AbTCR-CSR T cells was calculated by comparison with mock T cells. HLA-A2, and CD33 expression were measured by flow cytometric analysis.

WT1 expression was assessed by ESK1 and ESK2 staining. AML-14 is a positive control AML cell line.

+, ≥1.5-fold increase over the isotype control staining for WT1 RMF/HLA-A2; +, ≥20 fold over isotype for HLA-A2 and CD33; and none, 0% to 1.5% reduction over mock T-cell groups.

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