Key sensory neuron gene qRT-PCR targets and any known associations with SCD
| Target . | Protein . | Function . | Association with SCD . | Reference . |
|---|---|---|---|---|
| CACNA2D1 | Calcium channel subunit | Voltage-gated calcium channel | Potentially involved in spontaneous pain in SCD | 96 |
| CCL2 | Cytokine | Recruitment of immune cells | Upregulated during SCD | 55,97 |
| CCR1 | Chemokine receptor | Recruitment of immune cells | Differentially expressed in SCD | 56 |
| CCR2 | Chemokine receptor | Recruitment of immune cells | Contributes to hypersensitivity in SCD | 53 |
| CCR3 | Chemokine receptor | Recruitment of immune cells | None reported | — |
| CCR5 | Chemokine receptor | Recruitment of immune cells | Immune/inflammatory response in SCD | 57 |
| CD4 | Glycoprotein | T-cell co-receptor | Decreased in SCD | 98 |
| CGRP | Neuropeptide | Vasodilator | Upregulated during SCD | 23,99,100 |
| CHRNA9 | Ion channel subunit | Ligand-gated ion channel | None reported | — |
| CRLF2 | Cytokine receptor | Cell proliferation | None reported | — |
| CSF2Ra | Cytokine receptor subunit | Production of hematopoietic cells | None reported | — |
| CSF2Rb | Cytokine receptor subunit | Production of hematopoietic cells | Hydroxyurea metabolism | 101 |
| ETRa | Endothelin receptor | Vasoconstriction | Elevated in SCD; Contributes to hypersensitivity | 64,102 |
| ETRb | Endothelin receptor | Vasoconstriction | Red blood cell dehydration in SCD | 103 |
| HCN1 | Membrane channel | Hyperpolarization-activated cation channel | None reported | — |
| Nav1.7 | Sodium channel | Voltage-gated sodium channel | Associated with pain pathways | 104 |
| NF200 | Intermediate filament | Neuronal cytoskeleton | Associated with sensory neurons | 105 |
| P2X3 | Ion channel | Ligand-gated ion channel | Associated with sensory neurons | 105 |
| P2X4 | Ion channel | Ligand-gated ion channel | Speculated to be involved in red blood cell activity | 106 |
| Piezo1 | Ion channel | Mechanosensitive ion channel | Red blood cell dehydration in SCD | 107 |
| Piezo2 | Ion channel | Mechanosensitive ion channel | None reported | — |
| TRPA1 | Ion channel | Nonselective cation channel | Associated with pain in SCD | 108 |
| TRPC5 | Ion channel | Nonselective cation channel | Contributes to hypersensitivity in SCD | 105 |
| TRPM3 | Ion channel | Nonselective cation channel | None reported | — |
| TRPM8 | Ion channel | Nonselective cation channel | Speculated to be involved in hypersensitivity in SCD | 27 |
| TRPV1 | Calcium channel | Nonselective cation channel | Contributes to hypersensitivity in SCD | 24 |
| TRPV4 | Calcium channel | Nonselective cation channel | Contributes to hypersensitivity in SCD | 109 |
| Target . | Protein . | Function . | Association with SCD . | Reference . |
|---|---|---|---|---|
| CACNA2D1 | Calcium channel subunit | Voltage-gated calcium channel | Potentially involved in spontaneous pain in SCD | 96 |
| CCL2 | Cytokine | Recruitment of immune cells | Upregulated during SCD | 55,97 |
| CCR1 | Chemokine receptor | Recruitment of immune cells | Differentially expressed in SCD | 56 |
| CCR2 | Chemokine receptor | Recruitment of immune cells | Contributes to hypersensitivity in SCD | 53 |
| CCR3 | Chemokine receptor | Recruitment of immune cells | None reported | — |
| CCR5 | Chemokine receptor | Recruitment of immune cells | Immune/inflammatory response in SCD | 57 |
| CD4 | Glycoprotein | T-cell co-receptor | Decreased in SCD | 98 |
| CGRP | Neuropeptide | Vasodilator | Upregulated during SCD | 23,99,100 |
| CHRNA9 | Ion channel subunit | Ligand-gated ion channel | None reported | — |
| CRLF2 | Cytokine receptor | Cell proliferation | None reported | — |
| CSF2Ra | Cytokine receptor subunit | Production of hematopoietic cells | None reported | — |
| CSF2Rb | Cytokine receptor subunit | Production of hematopoietic cells | Hydroxyurea metabolism | 101 |
| ETRa | Endothelin receptor | Vasoconstriction | Elevated in SCD; Contributes to hypersensitivity | 64,102 |
| ETRb | Endothelin receptor | Vasoconstriction | Red blood cell dehydration in SCD | 103 |
| HCN1 | Membrane channel | Hyperpolarization-activated cation channel | None reported | — |
| Nav1.7 | Sodium channel | Voltage-gated sodium channel | Associated with pain pathways | 104 |
| NF200 | Intermediate filament | Neuronal cytoskeleton | Associated with sensory neurons | 105 |
| P2X3 | Ion channel | Ligand-gated ion channel | Associated with sensory neurons | 105 |
| P2X4 | Ion channel | Ligand-gated ion channel | Speculated to be involved in red blood cell activity | 106 |
| Piezo1 | Ion channel | Mechanosensitive ion channel | Red blood cell dehydration in SCD | 107 |
| Piezo2 | Ion channel | Mechanosensitive ion channel | None reported | — |
| TRPA1 | Ion channel | Nonselective cation channel | Associated with pain in SCD | 108 |
| TRPC5 | Ion channel | Nonselective cation channel | Contributes to hypersensitivity in SCD | 105 |
| TRPM3 | Ion channel | Nonselective cation channel | None reported | — |
| TRPM8 | Ion channel | Nonselective cation channel | Speculated to be involved in hypersensitivity in SCD | 27 |
| TRPV1 | Calcium channel | Nonselective cation channel | Contributes to hypersensitivity in SCD | 24 |
| TRPV4 | Calcium channel | Nonselective cation channel | Contributes to hypersensitivity in SCD | 109 |