Key sensory neuron gene transcript expression analyzed by qRT-PCR for HC and SCD iSNs
| Target . | HC iSN expression (mean ± SE) . | SCD iSN expression (mean ± SE) . | t test (P value) . |
|---|---|---|---|
| Cytokines and receptors | |||
| CCR1 | 1.1303 ± 0.8083 | 1.3154 ± 1.0460 | .8905 |
| CCR2 | 3.1287 ± 3.1055 | 2.2507 ± 1.9219 | .8137 |
| CCR3 | 0.6707 ± 0.5664 | 1.7838 ± 1.7412 | .5573 |
| CCR5 | 1.3152 ± 0.8065 | 0.8818 ± 0.4786 | .6516 |
| CCL2 | 0.0186 ± 0.5340 | 1.0975 ± 0.6934 | .2366 |
| Immune response and vasoconstriction | |||
| CD4 | 0.7275 ± 0.3015 | 1.1605 ± 0.7263 | .5932 |
| CRLF2 | 1.6009 ± 1.1369 | 1.3079 ± 0.7801 | .8347 |
| CSF2Ra | 0.4063 ± 0.1305 | 0.7119 ± 0.5496 | .6019 |
| CSF2Rb | 0.0385 ± 0.2229 | 0.3847 ± 1.2263 | .7878 |
| ETRa | 0.1256 ± 0.6420 | 0.6931 ± 0.6029 | .5285 |
| ETRb | 2.1103 ± 1.7300 | 1.8979 ± 1.5764 | .9288 |
| Ion channels: nociceptor relevant | |||
| HCN1 | 0.7730 ± 0.0928 | 0.7844 ± 0.3887 | .9779 |
| Nav1.7 | 0.6744 ± 0.0729 | 0.5603 ± 0.3913 | .7813 |
| P2X3 | 1.7744 ± 1.4308 | 1.4527 ± 1.0147 | .8570 |
| P2X4 | 0.2109 ± 0.1133 | 0.1079 ± 0.8384 | .9059 |
| CACNA2D1 | 0.3918 ± 0.0937 | 0.0371 ± 0.6868 | .6222 |
| CHRNA9 | 1.8654 ± 1.3584 | 1.6363 ± 1.0630 | .8960 |
| Ion channels: SCD pain specific | |||
| Piezo1 | 0.4244 ± 0.6581 | 0.5183 ± 1.0204 | .9395 |
| Piezo2 | 0.8643 ± 0.6382 | 0.9337 ± 0.7513 | .7360 |
| TRPA1 | 2.7452 ± 2.7365 | 1.6610 ± 1.5499 | .7360 |
| TRPC5 | 2.2992 ± 1.5932 | 2.8119 ± 2.5534 | .8673 |
| TRPM3 | 1.6454 ± 1.1363 | 1.5149 ± 0.9201 | .9301 |
| TRPM8 | 0.0087 ± 0.7276 | 0.0028 ± 0.5776 | .9951 |
| TRPV1 | 0.5636 ± 0.0269 | 0.7488 ± 0.2250 | .4368 |
| TRPV4 | 0.5997 ± 0.1999 | 0.9690 ± 0.5988 | .5718 |
| Target . | HC iSN expression (mean ± SE) . | SCD iSN expression (mean ± SE) . | t test (P value) . |
|---|---|---|---|
| Cytokines and receptors | |||
| CCR1 | 1.1303 ± 0.8083 | 1.3154 ± 1.0460 | .8905 |
| CCR2 | 3.1287 ± 3.1055 | 2.2507 ± 1.9219 | .8137 |
| CCR3 | 0.6707 ± 0.5664 | 1.7838 ± 1.7412 | .5573 |
| CCR5 | 1.3152 ± 0.8065 | 0.8818 ± 0.4786 | .6516 |
| CCL2 | 0.0186 ± 0.5340 | 1.0975 ± 0.6934 | .2366 |
| Immune response and vasoconstriction | |||
| CD4 | 0.7275 ± 0.3015 | 1.1605 ± 0.7263 | .5932 |
| CRLF2 | 1.6009 ± 1.1369 | 1.3079 ± 0.7801 | .8347 |
| CSF2Ra | 0.4063 ± 0.1305 | 0.7119 ± 0.5496 | .6019 |
| CSF2Rb | 0.0385 ± 0.2229 | 0.3847 ± 1.2263 | .7878 |
| ETRa | 0.1256 ± 0.6420 | 0.6931 ± 0.6029 | .5285 |
| ETRb | 2.1103 ± 1.7300 | 1.8979 ± 1.5764 | .9288 |
| Ion channels: nociceptor relevant | |||
| HCN1 | 0.7730 ± 0.0928 | 0.7844 ± 0.3887 | .9779 |
| Nav1.7 | 0.6744 ± 0.0729 | 0.5603 ± 0.3913 | .7813 |
| P2X3 | 1.7744 ± 1.4308 | 1.4527 ± 1.0147 | .8570 |
| P2X4 | 0.2109 ± 0.1133 | 0.1079 ± 0.8384 | .9059 |
| CACNA2D1 | 0.3918 ± 0.0937 | 0.0371 ± 0.6868 | .6222 |
| CHRNA9 | 1.8654 ± 1.3584 | 1.6363 ± 1.0630 | .8960 |
| Ion channels: SCD pain specific | |||
| Piezo1 | 0.4244 ± 0.6581 | 0.5183 ± 1.0204 | .9395 |
| Piezo2 | 0.8643 ± 0.6382 | 0.9337 ± 0.7513 | .7360 |
| TRPA1 | 2.7452 ± 2.7365 | 1.6610 ± 1.5499 | .7360 |
| TRPC5 | 2.2992 ± 1.5932 | 2.8119 ± 2.5534 | .8673 |
| TRPM3 | 1.6454 ± 1.1363 | 1.5149 ± 0.9201 | .9301 |
| TRPM8 | 0.0087 ± 0.7276 | 0.0028 ± 0.5776 | .9951 |
| TRPV1 | 0.5636 ± 0.0269 | 0.7488 ± 0.2250 | .4368 |
| TRPV4 | 0.5997 ± 0.1999 | 0.9690 ± 0.5988 | .5718 |
No significant differences in transcript expression of cytokine receptors, immune response and vasoconstriction related receptors, and ion channels were identified between HC and SCD iSNs as analyzed by qRT-PCR. Values for each target are normalized to housekeeping gene (glyceraldehyde-3-phosphate dehydrogenase) and Cq values for each sample were normalized to a single HC sample value to calculate relative fold change (ΔΔCq) in gene expression. Independent t tests for each target were used to analyze differences in expression between HC and SCD samples.
SE, standard error.