Summary of the key properties of GPVI Affimers
| Affimer . | Binding sites on GPVI . | KD GPVI monomer, nM . | KD GPVI dimer, nM . | Platelet aggregation (agonist: collagen/CRP) . | Thrombus formation under flow (collagen surface) . |
|---|---|---|---|---|---|
| M17 | 141Y-149T | 55 ± 18 | 4.4 ± 4.3 | ↓/↓ | — |
| D22 | 44S-53L | 53 ± 11 | 5.3 ± 2.5 | ↓/↓ | ↓ |
| D18 | 113Q-123F 141Y-149T | N.D. | 0.23 ± 0.01 | -/↓ | — |
| Affimer . | Binding sites on GPVI . | KD GPVI monomer, nM . | KD GPVI dimer, nM . | Platelet aggregation (agonist: collagen/CRP) . | Thrombus formation under flow (collagen surface) . |
|---|---|---|---|---|---|
| M17 | 141Y-149T | 55 ± 18 | 4.4 ± 4.3 | ↓/↓ | — |
| D22 | 44S-53L | 53 ± 11 | 5.3 ± 2.5 | ↓/↓ | ↓ |
| D18 | 113Q-123F 141Y-149T | N.D. | 0.23 ± 0.01 | -/↓ | — |
Down arrow (↓) represents inhibition effect. Hyphen (-) represents no clear inhibition effect. KD values were determined using SPR.
N.D., not determinable.