Table 1.

Clinical features of patients treated with HA-1 TCR-T

ParticipantAge, yDiseaseCytogenetic/molecularHCT to first relapse,
d
HCT to TCR-T, dDisease stageLymphodepletion (doses)TCR-T dose levelCRSICANSGVHD after TCR-TOther relevant AE (maximal grade)Disease outcome
27 B-ALL Ph-like
RCSD1-ABL2
+ complex 
987 >1000 1. MRD CR
2. MRD+ CR
3. MRD+ CR 
1. Fludarabine (3)
2. Fludarabine (3)
3. Fludarabine (3) 
1. DL1
2. DL2
3. DL2 
No No No No 1. Maintained CR 6 mo
2. Progressive disease
3. Progressive disease 
58 AML del 5, -7, other 195 257 MRD CR Fludarabine (3) DL1 No No No Neutropenia (4)  Progressive disease 
T-ALL t(8:14), biallelic loss of CDKN2A and CDKN2B 71 1. 181
2. 236
3. 546 
1. MRD+ CR
2. MRD+ CR
3. Relapse 
1. Fludarabine (2)
2. Nil
3. NECTAR 
1. DL1
2. DL2
3. DL2 
No No No Neutropenia (4), fatigue (2)  1. Response with MRD
2. MRD CR
3. Progressive disease 
64 MDS-AML -5, -7, complex 134 177 MRD+ CR Fludarabine (2) DL1 No No Grade 2 stage 1 acute GI Infection (3-5),  Progressive disease 
51 AML t(2:8)
U2AF1, IDH1 
381 1. 453
2. 557 
1. MRD+ CR
2. MRD+ CR 
1. Fludarabine (3)
2. Nil 
1. DL2
2. DL2 
No No No Fever (1) d 0-1,
self-resolving 
Progressive disease 
62 AML Complex,
TP53 
83 161 MRD+ CR Fludarabine (1) DL2 No No No Fever (1) d 0-1, self-resolving, Neutropenia (3)  Progressive disease 
23 AUL Complex 184 after second HCT 267 after second HCT MRD CRi Fludarabine (3) DL2 No No No Neutropenia (4), infection-related fever (3) through day 3,§  Progressive disease 
66 MDS-EB1 Complex, -7, TP53 84 1. 179
2. 589 
MRD CRi Fludarabine (2) 1. DL3
2. DL2 
No No Grade 1 acute skin, subsequent mild chronic Fever (2) with rigors, transient nausea, d 0-1, self-resolving Maintained CR >27 mo 
57 AML t(11:19), KMT2a 30 204 Refractory relapse (38% blasts) Clofarabine and cytarabine DL3 No No No Infusion reaction (4)
Neutropenia (4)  
CRi at 3 and 5 wk Relapse at 12 wk 
ParticipantAge, yDiseaseCytogenetic/molecularHCT to first relapse,
d
HCT to TCR-T, dDisease stageLymphodepletion (doses)TCR-T dose levelCRSICANSGVHD after TCR-TOther relevant AE (maximal grade)Disease outcome
27 B-ALL Ph-like
RCSD1-ABL2
+ complex 
987 >1000 1. MRD CR
2. MRD+ CR
3. MRD+ CR 
1. Fludarabine (3)
2. Fludarabine (3)
3. Fludarabine (3) 
1. DL1
2. DL2
3. DL2 
No No No No 1. Maintained CR 6 mo
2. Progressive disease
3. Progressive disease 
58 AML del 5, -7, other 195 257 MRD CR Fludarabine (3) DL1 No No No Neutropenia (4)  Progressive disease 
T-ALL t(8:14), biallelic loss of CDKN2A and CDKN2B 71 1. 181
2. 236
3. 546 
1. MRD+ CR
2. MRD+ CR
3. Relapse 
1. Fludarabine (2)
2. Nil
3. NECTAR 
1. DL1
2. DL2
3. DL2 
No No No Neutropenia (4), fatigue (2)  1. Response with MRD
2. MRD CR
3. Progressive disease 
64 MDS-AML -5, -7, complex 134 177 MRD+ CR Fludarabine (2) DL1 No No Grade 2 stage 1 acute GI Infection (3-5),  Progressive disease 
51 AML t(2:8)
U2AF1, IDH1 
381 1. 453
2. 557 
1. MRD+ CR
2. MRD+ CR 
1. Fludarabine (3)
2. Nil 
1. DL2
2. DL2 
No No No Fever (1) d 0-1,
self-resolving 
Progressive disease 
62 AML Complex,
TP53 
83 161 MRD+ CR Fludarabine (1) DL2 No No No Fever (1) d 0-1, self-resolving, Neutropenia (3)  Progressive disease 
23 AUL Complex 184 after second HCT 267 after second HCT MRD CRi Fludarabine (3) DL2 No No No Neutropenia (4), infection-related fever (3) through day 3,§  Progressive disease 
66 MDS-EB1 Complex, -7, TP53 84 1. 179
2. 589 
MRD CRi Fludarabine (2) 1. DL3
2. DL2 
No No Grade 1 acute skin, subsequent mild chronic Fever (2) with rigors, transient nausea, d 0-1, self-resolving Maintained CR >27 mo 
57 AML t(11:19), KMT2a 30 204 Refractory relapse (38% blasts) Clofarabine and cytarabine DL3 No No No Infusion reaction (4)
Neutropenia (4)  
CRi at 3 and 5 wk Relapse at 12 wk 

AE, adverse events; AUL, acute undifferentiated leukemia; Cri, CR with incomplete hematologic recovery; EB, excess blasts; GI, gastrointestinal; NECTAR, nelarabine, etoposide, and cyclophosphamide; Ph, Philadelphia chromosome.

Unrelated to TCR T-cell infusion.

Possibly or probably related to TCR T-cell infusion. Note: Cytopenias were short-term and self-resolving unless they were caused by progressive leukemia.

Infection = environmentally-acquired, severe Legionella pneumonia ultimately led to death with multiorgan failure concurrent with progressive AML, not attributed to TCR T-cell infusion.

§

Infection = methicillin-susceptible Staphylococcus aureus pneumonia and septicemia.

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