List of primers used to perform qRT-PCR
| Gene name . | Primer sequence . |
|---|---|
| Clusterin _total_forward | 5′-CTA TCT GCG GGT CAC CA-3′ |
| Clusterin _total_reverse | 5′-CTC AGT GAC ACC GGA AGG AAC-3′ |
| Clusterin_1_ forward | 5′-GCG AGC AGA GCG CTA TAA AT-3′ |
| Clusterin_1_ reverse | 5′-GAT GCG GTC ACC ATT CAT C-3′ |
| Clusterin_2_ forward | 5′-AGA TGG ATT CGG TGT GAA GG-3′ |
| Clusterin_2_ reverse | 5′-GAT GCG GTC ACC ATT CAT C-3′ |
| 18S_forward | 5′-GTT CCG ACC ATA AAC GAT GCC-3′ |
| 18S_reverse | 5′-TGG TGG TGC CCT TCC GTC AAT-3′ |
| Clusterin | qHsaCED0043884 (Bio-Rad) |
| GRP78 | qHsaCED0001784 (Bio-Rad) |
| GRP94_forward | 5′-ACT GTT GAG GAG CCC ATG GAG-3′ |
| GRP94_reverse | 5′-GCT GAA GAG TCT CGC GGG AAA-3′ |
| Bax_forward | 5′-CCA GCA AAC TGG TGC TCA AG-3′ |
| Bax_reverse | 5′-GGA GGC TTG AGG AGT CTC AC-3′ |
| CASP-3_forward | 5′-TCT GGT TTT CGG TGG GTG TG-3′ |
| CASP-3_reverse | 5′-CGC TTC CAT GTA TGA TCT TTG-3′ |
| CASP-8_forward | 5′-CTG CTG GGG ATG GCC ACT GTG-3′ |
| CASP-8_reverse | 5′-TCG CCT CGA GGA CAT CGC TCT-3′ |
| CASP-9 | qHsaCED00444 (Bio-Rad) |
| p53_forward | 5′-GTG GAA GGA AAT TTG CGT-3′ |
| p53_reverse | 5′-CCA GTG TGA TGA TGG TGA-3′ |
| Gene name . | Primer sequence . |
|---|---|
| Clusterin _total_forward | 5′-CTA TCT GCG GGT CAC CA-3′ |
| Clusterin _total_reverse | 5′-CTC AGT GAC ACC GGA AGG AAC-3′ |
| Clusterin_1_ forward | 5′-GCG AGC AGA GCG CTA TAA AT-3′ |
| Clusterin_1_ reverse | 5′-GAT GCG GTC ACC ATT CAT C-3′ |
| Clusterin_2_ forward | 5′-AGA TGG ATT CGG TGT GAA GG-3′ |
| Clusterin_2_ reverse | 5′-GAT GCG GTC ACC ATT CAT C-3′ |
| 18S_forward | 5′-GTT CCG ACC ATA AAC GAT GCC-3′ |
| 18S_reverse | 5′-TGG TGG TGC CCT TCC GTC AAT-3′ |
| Clusterin | qHsaCED0043884 (Bio-Rad) |
| GRP78 | qHsaCED0001784 (Bio-Rad) |
| GRP94_forward | 5′-ACT GTT GAG GAG CCC ATG GAG-3′ |
| GRP94_reverse | 5′-GCT GAA GAG TCT CGC GGG AAA-3′ |
| Bax_forward | 5′-CCA GCA AAC TGG TGC TCA AG-3′ |
| Bax_reverse | 5′-GGA GGC TTG AGG AGT CTC AC-3′ |
| CASP-3_forward | 5′-TCT GGT TTT CGG TGG GTG TG-3′ |
| CASP-3_reverse | 5′-CGC TTC CAT GTA TGA TCT TTG-3′ |
| CASP-8_forward | 5′-CTG CTG GGG ATG GCC ACT GTG-3′ |
| CASP-8_reverse | 5′-TCG CCT CGA GGA CAT CGC TCT-3′ |
| CASP-9 | qHsaCED00444 (Bio-Rad) |
| p53_forward | 5′-GTG GAA GGA AAT TTG CGT-3′ |
| p53_reverse | 5′-CCA GTG TGA TGA TGG TGA-3′ |