Comparison of TF antigen and activity assays for measuring of TF in EVs in plasma samples
Sample . | Antigen assay . | Functional assays . | ||
|---|---|---|---|---|
| FCM . | Total . | With anti-TF antibody . | Without anti-TF antibody . | |
| High TF-positive EVs/high TF-negative EV (specificity) | 1 (0.5-1.5) n = 8 | 10.1 (0.9-39) n = 13 | 23.3 (6.7-39) n = 5 | 2.4 (1.3-8.9) n = 4 |
| High TF-positive EVs/low TF-positive EVs (sensitivity) | 1.6 (0.7-2.9) n = 8 | 2.4 (0.8-5.9) n = 16 | 2.9 (1.9-5.9) n = 9 | 1.1 (0.9-4.9) n = 5 |
| With LPS stimulation/without LPS stimulation (sensitivity) | 2.3 (1-4.1) n = 8 | 6.2 (0.9-36.6) n = 4 | 6.4 (4-26.1) n = 7 | 2.6 (1.6-32.9) n = 4 |
Sample . | Antigen assay . | Functional assays . | ||
|---|---|---|---|---|
| FCM . | Total . | With anti-TF antibody . | Without anti-TF antibody . | |
| High TF-positive EVs/high TF-negative EV (specificity) | 1 (0.5-1.5) n = 8 | 10.1 (0.9-39) n = 13 | 23.3 (6.7-39) n = 5 | 2.4 (1.3-8.9) n = 4 |
| High TF-positive EVs/low TF-positive EVs (sensitivity) | 1.6 (0.7-2.9) n = 8 | 2.4 (0.8-5.9) n = 16 | 2.9 (1.9-5.9) n = 9 | 1.1 (0.9-4.9) n = 5 |
| With LPS stimulation/without LPS stimulation (sensitivity) | 2.3 (1-4.1) n = 8 | 6.2 (0.9-36.6) n = 4 | 6.4 (4-26.1) n = 7 | 2.6 (1.6-32.9) n = 4 |
Results expressed as median (range); only includes data from assays with a denominator of >0; data are from Bonifay et al.142