Table 1.

Definition of red cell transfusion-dependence and anemia response criteria in MF

Clinical trialTDATRANon-TDAResponse criteria for TDAResponse criteria for non-TDA
SIMPLIFY-1
Phase 3 study momelotinib vs ruxolitinib in JAKi-naïve MF
Mesa et al19  
≥4 units or Hb <8 g/dL in the 8 wk before randomization Not defined Not meeting criteria for TDA TD rate at week 24 (proportion of patients who were TD)
Transfusion rate (average number of units per subject-month) 
TI rate at week 24 (proportion of patients who were TI)
No transfusions and all Hb ≥8 g/dL in the prior 12 wk leading up to week 24 
SIMPLIFY-2
Phase 3 study momelotinib vs best available therapy in JAKi-treated MF
Harrison et al20  
MOMENTUM
Phase 3 study momelotinib vs danazol in JAKi-treated MF
Verstovsek et al21  
≥4 units in the 8 wk before randomization with each transfusion trigger of Hb ≤9.5 g/dL 1-3 units in the 8 wk before randomization Hb <10 g/dL without transfusions in the 8 wk before randomization TI rate at week 24
No transfusion and all Hb ≥8 g/dL in the prior 12 wk
Transfusion rate (average number of units per subject-month) 
≥2 g/dL increase in Hb over a rolling ≥12 consecutive weeks 
PERSIST-2
Phase 3 study pacritinib vs best available therapy in JAKi-treated MF
Mascarenhas et al22  
≥2 units per month in the 12 wk before cycle 1 d 1 Not defined Hb <10 g/dL without transfusions in the 12 wk before cycle 1 d 1 TI rate at week 24
Absence of transfusions in the prior 12 wk 
≥2 g/dL increase in Hb for ≥8 wk before week 24
Applicable to patients with baseline Hb <10 g/dL 
ACE-536-MF-001
Phase 2 study luspatercept in anemia-related to MF
Gerds et a11  
4-12 units in 12 wk before cycle 1 d 1
Transfusion threshold symptomatic anemia (Hb ≤9.5 g/dL) 
Not defined Hb ≤9.5 g/dL on ≥3 different days and without transfusions in 12 wk before cycle 1 d 1 Absence of transfusions for ≥12 wk ≥1.5 g/dL increase in baseline Hb for ≥12 wk without transfusion 
Established anemia response criteria      
GALE
Gale et al23  
≥2 units per month in the prior 12 wk Not defined No transfusions in the prior 12 wk No transfusions over any 12-wk interval with no minimum Hb requirement No transfusions over any 12-wk interval with no minimum Hb requirement 
IWG-MRT
Tefferi et al18  
≥6 units for Hb <8.5 g/dL in the 12 wk before screening
Most recent transfusion within 28 d before screening 
Not defined Not meeting criteria for TDA No transfusions over a rolling ≥12 consecutive weeks, +Hb ≥8.5 g ≥2 g/dL increase in baseline Hb
If transfused in prior month, pretransfusion Hb used as baseline
Applicable to patients with baseline Hb <10 g/dL 
Clinical trialTDATRANon-TDAResponse criteria for TDAResponse criteria for non-TDA
SIMPLIFY-1
Phase 3 study momelotinib vs ruxolitinib in JAKi-naïve MF
Mesa et al19  
≥4 units or Hb <8 g/dL in the 8 wk before randomization Not defined Not meeting criteria for TDA TD rate at week 24 (proportion of patients who were TD)
Transfusion rate (average number of units per subject-month) 
TI rate at week 24 (proportion of patients who were TI)
No transfusions and all Hb ≥8 g/dL in the prior 12 wk leading up to week 24 
SIMPLIFY-2
Phase 3 study momelotinib vs best available therapy in JAKi-treated MF
Harrison et al20  
MOMENTUM
Phase 3 study momelotinib vs danazol in JAKi-treated MF
Verstovsek et al21  
≥4 units in the 8 wk before randomization with each transfusion trigger of Hb ≤9.5 g/dL 1-3 units in the 8 wk before randomization Hb <10 g/dL without transfusions in the 8 wk before randomization TI rate at week 24
No transfusion and all Hb ≥8 g/dL in the prior 12 wk
Transfusion rate (average number of units per subject-month) 
≥2 g/dL increase in Hb over a rolling ≥12 consecutive weeks 
PERSIST-2
Phase 3 study pacritinib vs best available therapy in JAKi-treated MF
Mascarenhas et al22  
≥2 units per month in the 12 wk before cycle 1 d 1 Not defined Hb <10 g/dL without transfusions in the 12 wk before cycle 1 d 1 TI rate at week 24
Absence of transfusions in the prior 12 wk 
≥2 g/dL increase in Hb for ≥8 wk before week 24
Applicable to patients with baseline Hb <10 g/dL 
ACE-536-MF-001
Phase 2 study luspatercept in anemia-related to MF
Gerds et a11  
4-12 units in 12 wk before cycle 1 d 1
Transfusion threshold symptomatic anemia (Hb ≤9.5 g/dL) 
Not defined Hb ≤9.5 g/dL on ≥3 different days and without transfusions in 12 wk before cycle 1 d 1 Absence of transfusions for ≥12 wk ≥1.5 g/dL increase in baseline Hb for ≥12 wk without transfusion 
Established anemia response criteria      
GALE
Gale et al23  
≥2 units per month in the prior 12 wk Not defined No transfusions in the prior 12 wk No transfusions over any 12-wk interval with no minimum Hb requirement No transfusions over any 12-wk interval with no minimum Hb requirement 
IWG-MRT
Tefferi et al18  
≥6 units for Hb <8.5 g/dL in the 12 wk before screening
Most recent transfusion within 28 d before screening 
Not defined Not meeting criteria for TDA No transfusions over a rolling ≥12 consecutive weeks, +Hb ≥8.5 g ≥2 g/dL increase in baseline Hb
If transfused in prior month, pretransfusion Hb used as baseline
Applicable to patients with baseline Hb <10 g/dL 

IWG-MRT, IWG for Myelofibrosis Research and Treatment; TD, transfusion-dependent; TI, transfusion independent; TRA, transfusion-requiring anemia.

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