Putative QGRS within RP11-350G8.5 lncRNA, their position, results of thermodynamic ensemble prediction analyzed by RNAfold (ΔG), and T1/2 values for the oligonucleotides in 10 mM Tris-HCl buffer (pH 7.0) containing 100 mM KCl, obtained by CD melting experiments at 0.2°C/min
| QGRS . | Position . | ΔG, kcal/mol . | T1/2, °C . |
|---|---|---|---|
| GGCCAGGCAUGGACGG | 202 | −2.37 | 50.0 (±0.2) |
| GGCCAGGCCGGACGUGG | 840 | −3.04 | 58.8 (±0.2) |
| GGGAGGCCAAGGUGGG | 885 | −1.91 | 58.7 (±0.2) |
| GGAGGCGGAGG | 1051 | 0 | 59.4 (±0.4) |
| GGUGAGGUCUGGCUCCUGG | 1421 | −3.34 | 57.4 (±0.4) |
| QGRS . | Position . | ΔG, kcal/mol . | T1/2, °C . |
|---|---|---|---|
| GGCCAGGCAUGGACGG | 202 | −2.37 | 50.0 (±0.2) |
| GGCCAGGCCGGACGUGG | 840 | −3.04 | 58.8 (±0.2) |
| GGGAGGCCAAGGUGGG | 885 | −1.91 | 58.7 (±0.2) |
| GGAGGCGGAGG | 1051 | 0 | 59.4 (±0.4) |
| GGUGAGGUCUGGCUCCUGG | 1421 | −3.34 | 57.4 (±0.4) |
CD, circular dichroism; QGRS, quadruplex-forming G-rich sequences; T1/2, melting temperature.