Summary of testing with in vitro assays on longitudinal series of blood samples from BSE-infected recipient sheep
| Assay . | No. of positive samples/no. of samples tested (% positive samples) . | |||||
|---|---|---|---|---|---|---|
| TP1 6 mpi . | TP2 9 mpi . | TP3 12 mpi . | TP4 18-25∗ mpi . | TP5 32 mpi . | TP6 (terminal)15-36 mpi . | |
| mb-PMCA | 9/10 (90%) | 10/10 (100%) | 10/10 (100%) | 3/4 (75%) | 1/1 (100%) | 9/9 (100%) |
| capture-PMCA | 8/10 (80%) | 10/10 (100%) | 10/10 (100%) | 3/4 (75%) | 1/1 (100%) | 9/9 (100%) |
| WB IOME RT-QuIC | 7/10 (70%) | 8/10 (80%) | 9/10 (90%) | 3/4 (75%) | 1/1 (100%) | 6/9 (67%) |
| Assay . | No. of positive samples/no. of samples tested (% positive samples) . | |||||
|---|---|---|---|---|---|---|
| TP1 6 mpi . | TP2 9 mpi . | TP3 12 mpi . | TP4 18-25∗ mpi . | TP5 32 mpi . | TP6 (terminal)15-36 mpi . | |
| mb-PMCA | 9/10 (90%) | 10/10 (100%) | 10/10 (100%) | 3/4 (75%) | 1/1 (100%) | 9/9 (100%) |
| capture-PMCA | 8/10 (80%) | 10/10 (100%) | 10/10 (100%) | 3/4 (75%) | 1/1 (100%) | 9/9 (100%) |
| WB IOME RT-QuIC | 7/10 (70%) | 8/10 (80%) | 9/10 (90%) | 3/4 (75%) | 1/1 (100%) | 6/9 (67%) |
Please note time points TP1-TP5 are preclinical: clinical signs of BSE were evident only at TP6 (terminal).
Twenty-five mpi sample from sheep P453 tested negative by all 3 in vitro assays, despite earlier and subsequent time points testing positive (also see supplemental Figures 2-4); the reason for this apparently anomalous result could not be determined.