Comparison of transfusion transmission outcomes in sheep with results of in vitro assays and blood infectivity titers
| Results from sheep transfusion experiments . | Results of in vitro assays . | Results of mouse bioassay∗ . | ||||||
|---|---|---|---|---|---|---|---|---|
| Donor sheep ID† . | Transfusion outcome . | No. of recipients infected/no. of recipients transfused‡ . | Donor survival period, mpi§ . | mb-PMCA . | capture-PMCA . | WB IOME RT-QuIC . | No. of positive mice/no. of mice inoculated . | Log10 infectivity titers/mL WB (95% CI) . |
| N157 | T | 2/7 | 20 | +/− | + | − | ND | ND |
| N231 | T | 1/7 | 20 | +/− | + | − | ND | ND |
| N251 | T | 5/5 | 18 | + | + | + | ND | ND |
| N261 | T | 6/7 | 19 | + | + | + | ND | ND |
| N257 | T | 5/5 | 20 | + | ND | + | ND | ND |
| N232 | T | 5/7 | 21 | + | ND | − | 1/12 | 2.2 (1.7-2.6) |
| N236 | T | 5/5 | 12 | + | ND | + | 9/12 | 3.5 (3.1-3.9)|| |
| N226 | T | 1/7 | 28 | − | ND | − | ND | ND |
| N180 | T | 3/7 | 27 | + | ND | − | 0/12 | 0 (0-2.3) |
| N218 | T | 1/5 | 28 | − | ND | − | 0/11 | 0 (0-2.5)|| |
| N204 | T | 2/7 | 37 | + | ND | − | 0/12 | 0 (0-2.3) |
| N245 | T | 1/4 | 32 | + | ND | + | 9/12 | 2.8 (2.6-3.2)|| |
| N196 | NT | 0/6 | 20 | − | − | − | 0/12 | 0 (0-2.3) |
| N258 | NT | 0/7 | 31 | − | − | − | ND | ND |
| N188 | NT | 0/5 | 22 | − | − | − | ND | ND |
| N220 | NT | 0/5 | 26 | − | − | − | 0/12 | 0 (0-2.3) |
| N228 | NT | 0/4 | 31 | − | − | − | ND | ND |
| N234 | NT | 0/5 | 36 | − | − | − | 1/12 | 2.2 (1.6-2.6) |
| N246 | NT | 0/7 | 46 | − | − | − | ND | ND |
| N161 | NT | 0/7 | 39 | +/− | − | − | ND | ND |
| N239 | Negative control | NA | NA | − | ND | − | 0/12 | 0 (0-2.4) |
| N170 | Negative control | NA | NA | − | ND | − | ND | ND |
| N214 | Negative control | NA | NA | − | ND | − | ND | ND |
| N173 | Negative control | NA | NA | − | − | − | ND | ND |
| N217 | Negative control | NA | NA | − | − | − | ND | ND |
| N256 | Negative control | NA | NA | − | − | − | ND | ND |
| N155 | Negative control | NA | NA | − | − | − | ND | ND |
| N209 | Negative control | NA | NA | − | − | − | ND | ND |
| N152 | Negative control | NA | NA | − | − | − | ND | ND |
| Results from sheep transfusion experiments . | Results of in vitro assays . | Results of mouse bioassay∗ . | ||||||
|---|---|---|---|---|---|---|---|---|
| Donor sheep ID† . | Transfusion outcome . | No. of recipients infected/no. of recipients transfused‡ . | Donor survival period, mpi§ . | mb-PMCA . | capture-PMCA . | WB IOME RT-QuIC . | No. of positive mice/no. of mice inoculated . | Log10 infectivity titers/mL WB (95% CI) . |
| N157 | T | 2/7 | 20 | +/− | + | − | ND | ND |
| N231 | T | 1/7 | 20 | +/− | + | − | ND | ND |
| N251 | T | 5/5 | 18 | + | + | + | ND | ND |
| N261 | T | 6/7 | 19 | + | + | + | ND | ND |
| N257 | T | 5/5 | 20 | + | ND | + | ND | ND |
| N232 | T | 5/7 | 21 | + | ND | − | 1/12 | 2.2 (1.7-2.6) |
| N236 | T | 5/5 | 12 | + | ND | + | 9/12 | 3.5 (3.1-3.9)|| |
| N226 | T | 1/7 | 28 | − | ND | − | ND | ND |
| N180 | T | 3/7 | 27 | + | ND | − | 0/12 | 0 (0-2.3) |
| N218 | T | 1/5 | 28 | − | ND | − | 0/11 | 0 (0-2.5)|| |
| N204 | T | 2/7 | 37 | + | ND | − | 0/12 | 0 (0-2.3) |
| N245 | T | 1/4 | 32 | + | ND | + | 9/12 | 2.8 (2.6-3.2)|| |
| N196 | NT | 0/6 | 20 | − | − | − | 0/12 | 0 (0-2.3) |
| N258 | NT | 0/7 | 31 | − | − | − | ND | ND |
| N188 | NT | 0/5 | 22 | − | − | − | ND | ND |
| N220 | NT | 0/5 | 26 | − | − | − | 0/12 | 0 (0-2.3) |
| N228 | NT | 0/4 | 31 | − | − | − | ND | ND |
| N234 | NT | 0/5 | 36 | − | − | − | 1/12 | 2.2 (1.6-2.6) |
| N246 | NT | 0/7 | 46 | − | − | − | ND | ND |
| N161 | NT | 0/7 | 39 | +/− | − | − | ND | ND |
| N239 | Negative control | NA | NA | − | ND | − | 0/12 | 0 (0-2.4) |
| N170 | Negative control | NA | NA | − | ND | − | ND | ND |
| N214 | Negative control | NA | NA | − | ND | − | ND | ND |
| N173 | Negative control | NA | NA | − | − | − | ND | ND |
| N217 | Negative control | NA | NA | − | − | − | ND | ND |
| N256 | Negative control | NA | NA | − | − | − | ND | ND |
| N155 | Negative control | NA | NA | − | − | − | ND | ND |
| N209 | Negative control | NA | NA | − | − | − | ND | ND |
| N152 | Negative control | NA | NA | − | − | − | ND | ND |
NT, no transmission; T, transmission (transmission of infection from BSE-infected donor to ≥1 transfusion recipients); +, positive; +/−, weak positive; −, negative.
TgBov mouse bioassay (intracerebral), log10 infectivity titer (ID50/mL WB).
Samples from N257, N232, N236, N226, N180, N218, N204, and N245 (underlined) included in the longitudinal blood series (see supplemental Figures 2-4).
Number of BSE-infected recipient sheep (clinically and/or pathologically positive)/the number of recipients of blood components from a single BSE-infected donor.
Survival period, interval between the date of infection, and the date of euthanasia (on reaching humane clinical end points).
Titers from 10 mpi time point in longitudinal blood series (also shown in Table 5).