Table 4.

Recommendations and considerations for assessments and interventions during the course of CAR-T

30 days prior to CAR-TEarly post-CAR-T (≤30 days)Prolonged post-CAR-T (30-90 days)Late post-CAR-T (≥90 days)
HBV Bacterial prophylaxis Pneumocystis jiroveciiprophylaxis Vaccinations 
Check HBsAg, HBcAb, or HBV DNA. If positive, 0.5  mg entecavir daily prior to LD, and continue for 12 months. Tenofovir is an acceptable alternative. Levofloxacin 500  mg daily or equivalent with ANC <500/uL, until recovery (ANC >500/uL). Start TMP-SMX daily, or equivalent, on day 30 for 6-12 months.
Alternatives can include atovaquone, dapsone, or pentamidine. 
Start COVID-19 and influenza vaccines at 3 months, other inactivated vaccines at 6 months, and live vaccines >1 year from CAR-T. 
HCV/HIV Fungal prophylaxis IVIg 
Check HCV serology with reflex RNA and HIV fourth-generation antigen/antibody combination immunoassay. If positive consult with infectious disease for antiviral regimens. Fluconazole 200  mg daily with ANC <500/uL until recovery (ANC >500/uL).
If the patient has history of prior BMT, requires steroids for >3 days, or has prolonged neutropenia beyond 28 days, start a mold-active azolea with ANC <500/uL until recovery (ANC >500/uL). 
If asymptomatic, assess IgG levels at day 30 and then every 3-6 months until consistently >400  mg/dL. If patient has recurrent sinopulmonary infections and IgG <400  mg/dL, give IVIg, 0.5  g/kg body weight, and reassess monthly for continued replacement need. 
HSV/VZV prophylaxis Prolonged neutropenia 
Start 800  mg acyclovir twice daily or 500  mg valacyclovir twice daily prior to LD, and continue for ≥6 months, or 6 months beyond last episode of HSV or VZV. Consider G-CSF if ANC <500/µL beyond day 14. 
Prolonged neutropenia 
COVID/flu 
Influenza and COVID-19 vaccination prior to apheresis. 
CMV monitoring 
Pursued in patients with prior BMT or BCMA-targeted CAR-T or those who receive steroids >3 days for 1 month post-cessation of immunosuppression. 
30 days prior to CAR-TEarly post-CAR-T (≤30 days)Prolonged post-CAR-T (30-90 days)Late post-CAR-T (≥90 days)
HBV Bacterial prophylaxis Pneumocystis jiroveciiprophylaxis Vaccinations 
Check HBsAg, HBcAb, or HBV DNA. If positive, 0.5  mg entecavir daily prior to LD, and continue for 12 months. Tenofovir is an acceptable alternative. Levofloxacin 500  mg daily or equivalent with ANC <500/uL, until recovery (ANC >500/uL). Start TMP-SMX daily, or equivalent, on day 30 for 6-12 months.
Alternatives can include atovaquone, dapsone, or pentamidine. 
Start COVID-19 and influenza vaccines at 3 months, other inactivated vaccines at 6 months, and live vaccines >1 year from CAR-T. 
HCV/HIV Fungal prophylaxis IVIg 
Check HCV serology with reflex RNA and HIV fourth-generation antigen/antibody combination immunoassay. If positive consult with infectious disease for antiviral regimens. Fluconazole 200  mg daily with ANC <500/uL until recovery (ANC >500/uL).
If the patient has history of prior BMT, requires steroids for >3 days, or has prolonged neutropenia beyond 28 days, start a mold-active azolea with ANC <500/uL until recovery (ANC >500/uL). 
If asymptomatic, assess IgG levels at day 30 and then every 3-6 months until consistently >400  mg/dL. If patient has recurrent sinopulmonary infections and IgG <400  mg/dL, give IVIg, 0.5  g/kg body weight, and reassess monthly for continued replacement need. 
HSV/VZV prophylaxis Prolonged neutropenia 
Start 800  mg acyclovir twice daily or 500  mg valacyclovir twice daily prior to LD, and continue for ≥6 months, or 6 months beyond last episode of HSV or VZV. Consider G-CSF if ANC <500/µL beyond day 14. 
Prolonged neutropenia 
COVID/flu 
Influenza and COVID-19 vaccination prior to apheresis. 
CMV monitoring 
Pursued in patients with prior BMT or BCMA-targeted CAR-T or those who receive steroids >3 days for 1 month post-cessation of immunosuppression. 
a

Mold-active azoles include voriconazole, posaconazole, or isavuconazole.

G-CSF, granulocyte colony-stimulating factor; TMP-SMX, trimethoprim-sulfamethoxazole.

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