Table 2.

Studies on the genetic changes occurring in AML at post-transplantation relapse

ReferenceDiseaseNAnalysis of macroalterationsMutational profiling
Bacher et al68  AML 26 Yes (by standard cytogenetics and FISH): new genomic macroalterations in 20/26 relapses No 
Waterhouse et al25  AML 21 Yes (by SNP arrays): new genomic macroalterations in 16/21 relapses, FLT3-ITD CN-LOH in 1/21 No 
Quek et al22  AML 29 Yes (by standard cytogenetics and FISH): new genomic macroalterations in 16/29 relapses Yes (by targeted NGS panel): changes in mutational profile in 13/29 relapses, new mutations in TET2, NRAS, WT1, ETV6, RUNX1, DNMT3A, TP53, NPM1, IDH1, FLT3 ITD, PHF6 
Christopher et al24  AML 15 No Yes (by WES): changes in mutational profile in 13/15 relapses, new mutations in NRAS, FLT3, WT1, STAG2 
Vosberg et al27  AML 12 No Yes (by WES): new WT1 mutations in 6/12 relapses 
Toffalori et al41  AML 12 Yes (by SNP arrays): new genomic macroalterations in 7/12 relapses, FLT3-ITD CN-LOH in 2/12 No 
Hong et al21  AML/MDS 49 No Yes (by targeted NGS panel): changes in mutational profile in 46/49 relapses, new IDH1 mutations 
Pagliuca et al69  AML/MDS 55 No Yes (by targeted NGS panel, including HLA genes): HLA mutations in 9/40 diagnoses and 17/44 relapses, variable changes in non–HLA genes 
Wienecke et al23  AML 59 No Yes (by WES): changes in mutational profile in 28/59 relapses, mutations in spliceosome and epigenetic modifiers stable, in signal transduction genes unstable 
ReferenceDiseaseNAnalysis of macroalterationsMutational profiling
Bacher et al68  AML 26 Yes (by standard cytogenetics and FISH): new genomic macroalterations in 20/26 relapses No 
Waterhouse et al25  AML 21 Yes (by SNP arrays): new genomic macroalterations in 16/21 relapses, FLT3-ITD CN-LOH in 1/21 No 
Quek et al22  AML 29 Yes (by standard cytogenetics and FISH): new genomic macroalterations in 16/29 relapses Yes (by targeted NGS panel): changes in mutational profile in 13/29 relapses, new mutations in TET2, NRAS, WT1, ETV6, RUNX1, DNMT3A, TP53, NPM1, IDH1, FLT3 ITD, PHF6 
Christopher et al24  AML 15 No Yes (by WES): changes in mutational profile in 13/15 relapses, new mutations in NRAS, FLT3, WT1, STAG2 
Vosberg et al27  AML 12 No Yes (by WES): new WT1 mutations in 6/12 relapses 
Toffalori et al41  AML 12 Yes (by SNP arrays): new genomic macroalterations in 7/12 relapses, FLT3-ITD CN-LOH in 2/12 No 
Hong et al21  AML/MDS 49 No Yes (by targeted NGS panel): changes in mutational profile in 46/49 relapses, new IDH1 mutations 
Pagliuca et al69  AML/MDS 55 No Yes (by targeted NGS panel, including HLA genes): HLA mutations in 9/40 diagnoses and 17/44 relapses, variable changes in non–HLA genes 
Wienecke et al23  AML 59 No Yes (by WES): changes in mutational profile in 28/59 relapses, mutations in spliceosome and epigenetic modifiers stable, in signal transduction genes unstable 

FISH, fluorescence in situ hybridization; NGS, next-generation sequencing; SNP, single nucleotide polymorphism; WES, whole-exome sequencing.

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