Representation of the mutations with higher VAFs for each gene; there are some cases where a gene has several different mutations with lower VAFs that are not represented in the table. See supplemental Table 1 for full data.

Bulk: mononuclear cells isolated from the peripheral blood of patients with AML.

CD34⁺: sorted populations from bulk samples, according to CD34⁺ expression.

Xeno: BM from each mouse engrafted with ITD-positive AML was depleted from murine cells, genomic DNA was isolated and targeted next-generation sequencing was performed (single-molecule molecular inversion probe panel and FLT3_ITD_ext algorithm, as explained in the text).

M1 to M5: mouse 1 to mouse 5 in each experimental condition. VAFs are represented in percentages.

FLT3-ITD mutation calling was considered positive if detected by the following 2 algorithms: FLT3_ITD_ext and PINDEL. VAFs were determined by FLT3_ITD_ext.

∗FLT3-KO mutation present in sample ITD 7 corresponds to the edited region of FLT3 upon KO. A complex myriad of insertions and deletions was found in FLT3-KO mice, but only the mutation with higher VAF is represented in the table for each mouse. See supplemental Table 1 for full data.