Rapid ADAMTS13 activity assays for TTP
| Rapid assay (manufacturer) . | HemosIL AcuStar ADAMTS13 activity (Werfen/Instrumentation Laboratory) . | Technofluor ADAMTS13 activity (Technoclone) . | Technoscreen ADAMTS13 activity (Technoclone) . |
|---|---|---|---|
| Substrate | GST-VWF73 peptide | VWF73-based substrate | GST-VWF73 |
| Detection method | CLIA | FRET assay | Kit with flow-through membrane with streptavidin-gold conjugate to generate a red color |
| Analyzer | ACL AcuStar/Bio-Flash (automated) | Ceveron s100 (automated) | Technician comparison of color intensity with reference color chart |
| Analytical TAT | 33 minutes | 25 minutes | Estimated 21-40 minutes |
| Assay range | Quantitative: 0.2%-150% | Quantitative: 1%-125% | Semiquantitative: 0%, 10%, 40%, or 80% |
| Sample (volume) | Citrate plasma (10 μL) | Citrate plasma (7 μL) | Citrate plasma (300 μL) |
| Rapid assay (manufacturer) . | HemosIL AcuStar ADAMTS13 activity (Werfen/Instrumentation Laboratory) . | Technofluor ADAMTS13 activity (Technoclone) . | Technoscreen ADAMTS13 activity (Technoclone) . |
|---|---|---|---|
| Substrate | GST-VWF73 peptide | VWF73-based substrate | GST-VWF73 |
| Detection method | CLIA | FRET assay | Kit with flow-through membrane with streptavidin-gold conjugate to generate a red color |
| Analyzer | ACL AcuStar/Bio-Flash (automated) | Ceveron s100 (automated) | Technician comparison of color intensity with reference color chart |
| Analytical TAT | 33 minutes | 25 minutes | Estimated 21-40 minutes |
| Assay range | Quantitative: 0.2%-150% | Quantitative: 1%-125% | Semiquantitative: 0%, 10%, 40%, or 80% |
| Sample (volume) | Citrate plasma (10 μL) | Citrate plasma (7 μL) | Citrate plasma (300 μL) |
GST-VWF73-His, glutathione S-transferase tagged VWF fragment.