FVIII disulfide state and disulfide bond conformation
| FVIII disulfide . | Fraction formed . | Conformation . |
|---|---|---|
| C172-C198 | 0.93 | –LHhook |
| C267-C348 | 0.63 | –LHhook |
| C547-C573 | 0.76 | –RHstaple |
| C649-C730 | 0.77 | ±RHstaple |
| C1851-C1877 | 0.81 | –LHstaple |
| C1918-C1922 | 0.55 | n.d.∗ |
| C2040-C2188 | 0.80 | ±RHhook |
| C2193-C2345 | 0.81 | ±RHhook |
| FVIII disulfide . | Fraction formed . | Conformation . |
|---|---|---|
| C172-C198 | 0.93 | –LHhook |
| C267-C348 | 0.63 | –LHhook |
| C547-C573 | 0.76 | –RHstaple |
| C649-C730 | 0.77 | ±RHstaple |
| C1851-C1877 | 0.81 | –LHstaple |
| C1918-C1922 | 0.55 | n.d.∗ |
| C2040-C2188 | 0.80 | ±RHhook |
| C2193-C2345 | 0.81 | ±RHhook |
The disulfide conformations were determined using the Disulfide Bond Analysis tool30 and Protein Data Bank identifier 7k66 structure of FVIII.31 The fraction formed are the mean values of 3 recombinant FVIII preparations (data from Figure 1C).
n.d., not determined.
This bond is not present in the 7k66 FVIII structure.