Table 2.

Overview of the impact of CD8 levels on HCT outcomes in children and adults

ReferencePlatformAdult/pediatricOutcomeNo. of patientsType of study
Yakoub-Agha et al46  Unmanipulated BMT and PBSC Adult, pediatric CD28neg CD8+ T cells < 179 cells per μL at day 60 associated with higher cumulative incidence of relapse 80, single center Prospective 
Tian et al47  Unmanipulated haplo Adult, pediatric ≥375 cells per μL at day 90 reduced infections, improved NRM, LFS, and OS 214, single center Prospective 
Ranti et al48  BMT, PBSC Adult >50 CD8+ T cells per μL reduces relapse incidence.
No effect on OS, DFS. 
120, single center Retrospective 
Bondanza et al49  Haplo (unmanipulated, CD34+, TCD) Adult, pediatric <20 CD8+ T cells per μL reduces OS and increases NRM 144, multicenter Retrospective 
Troullioud Lucas et al21  All Pediatric, young adult No association found between CD8+ T-cell IR and outcomes 503, multicenter Retrospective 
Huang et al13  All Adult, young adult In pts with early CD4+ T-cell recovery (>50/μL by day 100), higher CD8+ is associated with reduced EBV reactivation 122, single center Retrospective 
Belinovski et al44  All Pediatric On 180 day CI of CMV infection higher for patients CD3+CD8+ of ≥200/μL on day +100. 111, single center Retrospective 
Soares et al14  MUD Adult Early increase in CD8+ T-cell subsets in patients who later develop cGVHD 40, single center Prospective 
Ando et al12  All Adult Higher CD8+ T cells on day +100 reduces NRM;higher activated CD8+ T cells on day +100 increases cGVHD 358, single center Retrospective 
ReferencePlatformAdult/pediatricOutcomeNo. of patientsType of study
Yakoub-Agha et al46  Unmanipulated BMT and PBSC Adult, pediatric CD28neg CD8+ T cells < 179 cells per μL at day 60 associated with higher cumulative incidence of relapse 80, single center Prospective 
Tian et al47  Unmanipulated haplo Adult, pediatric ≥375 cells per μL at day 90 reduced infections, improved NRM, LFS, and OS 214, single center Prospective 
Ranti et al48  BMT, PBSC Adult >50 CD8+ T cells per μL reduces relapse incidence.
No effect on OS, DFS. 
120, single center Retrospective 
Bondanza et al49  Haplo (unmanipulated, CD34+, TCD) Adult, pediatric <20 CD8+ T cells per μL reduces OS and increases NRM 144, multicenter Retrospective 
Troullioud Lucas et al21  All Pediatric, young adult No association found between CD8+ T-cell IR and outcomes 503, multicenter Retrospective 
Huang et al13  All Adult, young adult In pts with early CD4+ T-cell recovery (>50/μL by day 100), higher CD8+ is associated with reduced EBV reactivation 122, single center Retrospective 
Belinovski et al44  All Pediatric On 180 day CI of CMV infection higher for patients CD3+CD8+ of ≥200/μL on day +100. 111, single center Retrospective 
Soares et al14  MUD Adult Early increase in CD8+ T-cell subsets in patients who later develop cGVHD 40, single center Prospective 
Ando et al12  All Adult Higher CD8+ T cells on day +100 reduces NRM;higher activated CD8+ T cells on day +100 increases cGVHD 358, single center Retrospective 

BMT, BM transplantation; CI, cumulative incidence; CMV, cytomegalovirus; DFS, disease-free survival; EBV, Epstein-Barr virus; haplo, haploidentical transplant; LFS, leukemia-free survival; MUD, matched unrelated donor; PBSC, peripheral blood mononuclear cell; pts, patients.

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