Antibodies and methods for immunohistochemical stains
Antibody . | Clone . | Source . | Dilution . | Retrieval . |
|---|---|---|---|---|
| CD3 | PS1 | Ventana Medical Systems, Tucson, AZ | Neat | A |
| CD5 | 4C7 | Novocastra, Newcastle upon Tyne, United Kingdom | 1:20 | A |
| CD20 | L26 | DAKO, Carpinteria, CA | 1:200 | A |
| CD23 | BU38 | The Binding Site, San Diego, CA | 1:5 | B |
| CD43 | L60 | Ventana Medical Systems | Neat | None |
| Cyclin D1 | DCS-6 | DAKO | 1:200 | A |
| Cyclin D1 | SP4 | Neomarkers | 1:100 | C |
| Cyclin D2 | Polyclonal | Santa Cruz Biotech, Santa Cruz, CA | 1:500 | D |
| Cyclin D3 | DCS-22 | Neomarkers | 1:100 | E |
| Cyclin E | 13A3 | Novocastra | 1:10 | E |
| RB | Rb1 | DAKO | 1:10 | A |
| p27Kip1 | SX53G8 | DAKO | 1:20 | A |
Antibody . | Clone . | Source . | Dilution . | Retrieval . |
|---|---|---|---|---|
| CD3 | PS1 | Ventana Medical Systems, Tucson, AZ | Neat | A |
| CD5 | 4C7 | Novocastra, Newcastle upon Tyne, United Kingdom | 1:20 | A |
| CD20 | L26 | DAKO, Carpinteria, CA | 1:200 | A |
| CD23 | BU38 | The Binding Site, San Diego, CA | 1:5 | B |
| CD43 | L60 | Ventana Medical Systems | Neat | None |
| Cyclin D1 | DCS-6 | DAKO | 1:200 | A |
| Cyclin D1 | SP4 | Neomarkers | 1:100 | C |
| Cyclin D2 | Polyclonal | Santa Cruz Biotech, Santa Cruz, CA | 1:500 | D |
| Cyclin D3 | DCS-22 | Neomarkers | 1:100 | E |
| Cyclin E | 13A3 | Novocastra | 1:10 | E |
| RB | Rb1 | DAKO | 1:10 | A |
| p27Kip1 | SX53G8 | DAKO | 1:20 | A |
A indicates 10 mM citrate buffer, pH 6.0, 30 minutes, water bath (95°C); B, protease I enzymatic digestion, 8 minutes; C, 1 mM EDTA (ethylenediaminetetraacetic acid, pH 8.0), 30 minutes, water bath (95°C); D, 1 mM EDTA, pH 8.0, 60 minutes, water bath (95°C); and E, 10 mM citrate buffer, pH 6.0, 10 minutes, pressure cooker (115°C).