Blood values and number of cells in femur and spleen from 12- to 13- and 16- to 20-month-old mice harboring the GATA-1low mutation in the C57BL/6, DBA/2, and CD1 backgrounds, as indicated
. | . | 12 to 13 months . | . | . | . | . | 16 to 20 months . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Blood . | . | . | Femur cells, × 106 . | Spleen cells, × 106 . | Blood . | . | . | Femur cells, × 106 . | Spleen cells, × 106 . | ||||||||
| Strain . | Genotype . | Hct, % . | Ptl, × 109/L . | WBC, × 106/L . | . | . | Hct, % . | Ptl, × 109/L . | WBC, × 106/L . | . | . | ||||||||
| C57BL/6 | |||||||||||||||||||
| F1 + F2 | Wild type | 44.8 ± 5.0 | 1.4 ± 0.2 | 11 ± 0.3 | 25.4 ± 7.3 | 114 ± 26 | 46.1 ± 1.8 | ND | ND | 28.8 ± 4.3 | 110 ± 11 | ||||||||
| F1 + F2 | GATA-1low/+ | 48.6 ± 7.0 | 0.4 ± 0.2* | 5.5 ± 2.1* | 9.9 ± 1.8* | 623 ± 532* | 40.2 ± 6.2 | 0.30 ± 0.02 | 3.2 ± 2.0 | 8.2 ± 2.2* | 364 ± 97* | ||||||||
| DBA/2 | |||||||||||||||||||
| F1 + F2 | Wild type | 44.0 ± 2.3 | 1.3 ± 0.3 | 8.0 ± 0.1 | 13.4 ± 4.7 | 71 ± 13 | 42.5 ± 1.3 | ND | ND | 8.8 ± 3.5 | 71 ± 14 | ||||||||
| F1 + F2 | GATA-1low/+ | 48.8 ± 3.2 | 0.42 ± 0.27* | 4.3 ± 1.4* | 2.2 ± 1.5* | 760 ± 141* | 35.0 ± 1.7* | 0.20 ± 0.05 | 9.3 ± 4.6 | 10.5 ± 4.2 | 265 ± 90* | ||||||||
| F2 | GATA-1low/0 | 36.6 ± 2.0* | 0.17 ± 0.25* | 4.0 ± 1.3* | 8.2 ± 2.8* | 133 ± 66 | 37.3 ± 2.6* | ND | ND | 2.5 ± 0.2* | 101 ± 19 | ||||||||
| CD1 | |||||||||||||||||||
| F1 + F2 | Wild type | 48.5 ± 3.4 | 0.9 ± 0.2 | 6.0 ± 0.2 | 15.7 ± 0.9 | 180 ± 45 | 45.3 ± 3.1 | 1.1 ± 0.3 | 7.1 ± 1.8 | 16.2 ± 1.8 | 100 ± 35 | ||||||||
| F1 + F2 | GATA-1low/+ | 45.0 ± 3.3 | 0.3 ± 0.1* | 3.9 ± 0.3* | 3.6 ± 3.0* | 1065 ± 318* | 43.0 ± 4.1 | 0.45 ± 0.2 | 5.4 ± 2.2 | ND | 101 ± 36 | ||||||||
| F2 | GATA-1low/0 | 45.0 ± 3.3 | 0.08 ± 0.03* | 8.1 ± 2.2* | 5.0 ± 2.1* | 670 ± 190* | 38.5 ± 2.7* | 0.09 ± 0.05 | 10.4 ± 3.0 | 3.8 ± 1.1* | 550 ± 170* | ||||||||
. | . | 12 to 13 months . | . | . | . | . | 16 to 20 months . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Blood . | . | . | Femur cells, × 106 . | Spleen cells, × 106 . | Blood . | . | . | Femur cells, × 106 . | Spleen cells, × 106 . | ||||||||
| Strain . | Genotype . | Hct, % . | Ptl, × 109/L . | WBC, × 106/L . | . | . | Hct, % . | Ptl, × 109/L . | WBC, × 106/L . | . | . | ||||||||
| C57BL/6 | |||||||||||||||||||
| F1 + F2 | Wild type | 44.8 ± 5.0 | 1.4 ± 0.2 | 11 ± 0.3 | 25.4 ± 7.3 | 114 ± 26 | 46.1 ± 1.8 | ND | ND | 28.8 ± 4.3 | 110 ± 11 | ||||||||
| F1 + F2 | GATA-1low/+ | 48.6 ± 7.0 | 0.4 ± 0.2* | 5.5 ± 2.1* | 9.9 ± 1.8* | 623 ± 532* | 40.2 ± 6.2 | 0.30 ± 0.02 | 3.2 ± 2.0 | 8.2 ± 2.2* | 364 ± 97* | ||||||||
| DBA/2 | |||||||||||||||||||
| F1 + F2 | Wild type | 44.0 ± 2.3 | 1.3 ± 0.3 | 8.0 ± 0.1 | 13.4 ± 4.7 | 71 ± 13 | 42.5 ± 1.3 | ND | ND | 8.8 ± 3.5 | 71 ± 14 | ||||||||
| F1 + F2 | GATA-1low/+ | 48.8 ± 3.2 | 0.42 ± 0.27* | 4.3 ± 1.4* | 2.2 ± 1.5* | 760 ± 141* | 35.0 ± 1.7* | 0.20 ± 0.05 | 9.3 ± 4.6 | 10.5 ± 4.2 | 265 ± 90* | ||||||||
| F2 | GATA-1low/0 | 36.6 ± 2.0* | 0.17 ± 0.25* | 4.0 ± 1.3* | 8.2 ± 2.8* | 133 ± 66 | 37.3 ± 2.6* | ND | ND | 2.5 ± 0.2* | 101 ± 19 | ||||||||
| CD1 | |||||||||||||||||||
| F1 + F2 | Wild type | 48.5 ± 3.4 | 0.9 ± 0.2 | 6.0 ± 0.2 | 15.7 ± 0.9 | 180 ± 45 | 45.3 ± 3.1 | 1.1 ± 0.3 | 7.1 ± 1.8 | 16.2 ± 1.8 | 100 ± 35 | ||||||||
| F1 + F2 | GATA-1low/+ | 45.0 ± 3.3 | 0.3 ± 0.1* | 3.9 ± 0.3* | 3.6 ± 3.0* | 1065 ± 318* | 43.0 ± 4.1 | 0.45 ± 0.2 | 5.4 ± 2.2 | ND | 101 ± 36 | ||||||||
| F2 | GATA-1low/0 | 45.0 ± 3.3 | 0.08 ± 0.03* | 8.1 ± 2.2* | 5.0 ± 2.1* | 670 ± 190* | 38.5 ± 2.7* | 0.09 ± 0.05 | 10.4 ± 3.0 | 3.8 ± 1.1* | 550 ± 170* | ||||||||
The values obtained in age-matched normal littermates are also reported for comparison. Data represent the means (±SD) of independent determinations on at least 4 to 10 mice per experimental group.
ND indicates not done.
Values statistically different (P < .01) from those observed in the corresponding wild-type littermates at the GATA-1 locus