Detection and quantification of HTLV-1 DNA in rabbit PBMCs
Inoculum and rabbit . | Weeks after inoculation . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 6 . | 8* . | ||||
| 729.HTLV-1 | |||||||||
| R1 | - | + (0.001) | + | + | + (0.112) | ||||
| R2 | - | + (0.001) | + | + | + (0.108) | ||||
| R3 | - | + (0.004) | + | + | + (0.122) | ||||
| R4 | - | + (0.013) | + | + | + (0.035) | ||||
| R5 | - | + (0.002) | + | + | + (0.053) | ||||
| 729.HTLV-1ΔHBZ | |||||||||
| R6 | - | + (0.0003) | + | + | + (0.002) | ||||
| R7 | - | + (0.0003) | + | + | + (0.004) | ||||
| R8 | - | + (0.0008) | + | + | + (0.007) | ||||
| R9 | - | + (0.0003) | + | + | + (0.001) | ||||
| R10 | - | + (0.0002) | + | + | + (0.001) | ||||
| R11 | - | + (0.0005) | + | + | + (0.013) | ||||
| 729.HTLV-1HBZΔLZ | |||||||||
| R12 | - | + (0.0003) | + | + | + (0.001) | ||||
| R13 | - | + (0.0002) | + | + | + (0.005) | ||||
| R14 | - | + (0.0002) | + | + | + (0.014) | ||||
| R15 | - | + (0.0002) | + | + | + (0.006) | ||||
| R16 | - | + (0.0003) | + | + | + (0.004) | ||||
| R17 | - | + (0.0001) | + | + | + (0.003) | ||||
| 729 | |||||||||
| R18 | - | - (0.0001) | - | - | - (0.0001) | ||||
| Media | |||||||||
| R19 | - | - (0.0001) | - | - | - (0.0001) | ||||
Inoculum and rabbit . | Weeks after inoculation . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| . | 0 . | 2 . | 4 . | 6 . | 8* . | ||||
| 729.HTLV-1 | |||||||||
| R1 | - | + (0.001) | + | + | + (0.112) | ||||
| R2 | - | + (0.001) | + | + | + (0.108) | ||||
| R3 | - | + (0.004) | + | + | + (0.122) | ||||
| R4 | - | + (0.013) | + | + | + (0.035) | ||||
| R5 | - | + (0.002) | + | + | + (0.053) | ||||
| 729.HTLV-1ΔHBZ | |||||||||
| R6 | - | + (0.0003) | + | + | + (0.002) | ||||
| R7 | - | + (0.0003) | + | + | + (0.004) | ||||
| R8 | - | + (0.0008) | + | + | + (0.007) | ||||
| R9 | - | + (0.0003) | + | + | + (0.001) | ||||
| R10 | - | + (0.0002) | + | + | + (0.001) | ||||
| R11 | - | + (0.0005) | + | + | + (0.013) | ||||
| 729.HTLV-1HBZΔLZ | |||||||||
| R12 | - | + (0.0003) | + | + | + (0.001) | ||||
| R13 | - | + (0.0002) | + | + | + (0.005) | ||||
| R14 | - | + (0.0002) | + | + | + (0.014) | ||||
| R15 | - | + (0.0002) | + | + | + (0.006) | ||||
| R16 | - | + (0.0003) | + | + | + (0.004) | ||||
| R17 | - | + (0.0001) | + | + | + (0.003) | ||||
| 729 | |||||||||
| R18 | - | - (0.0001) | - | - | - (0.0001) | ||||
| Media | |||||||||
| R19 | - | - (0.0001) | - | - | - (0.0001) | ||||
Genomic DNA was isolated from rabbit PBMCs (weeks 2 and 8 after inoculation) and subjected to real-time Taqman PCR using HTLV-1-specific primers (670/671). Numbers in parentheses at weeks 2 and 8 denote copy number per cell in rabbit PBMCs.
- indicates no amplified PCR fragment; +, amplified PCR fragment.
Copy numbers in rabbits inoculated with the mutant proviruses at week 8 were significantly different from wild type, as determined by ANOVA followed by Tukey test (P < .001).