Truncations and site-directed mutagenesis in the carboxyl terminus and 3i loop of CXCR3
CXCR3 receptor . | Surface binding of iodinated CXCL11 to CXCR3, % WT . | IC50, nM . |
|---|---|---|
| 293-WT | 100 | 8 |
| 293-349stopΔ20 | 70.4 | 8 |
| 293-332stopΔ37 | 71.2 | 6 |
| 293-332-334L→A | 70.0 | 4 |
| 293-S245A | 85.4 | 1 |
| Jurkat-WT | 100 | 0.4 |
| Jurkat-S245A | 115 | 0.2 |
CXCR3 receptor . | Surface binding of iodinated CXCL11 to CXCR3, % WT . | IC50, nM . |
|---|---|---|
| 293-WT | 100 | 8 |
| 293-349stopΔ20 | 70.4 | 8 |
| 293-332stopΔ37 | 71.2 | 6 |
| 293-332-334L→A | 70.0 | 4 |
| 293-S245A | 85.4 | 1 |
| Jurkat-WT | 100 | 0.4 |
| Jurkat-S245A | 115 | 0.2 |
Relative surface binding, to WT CXCR3, of iodinated CXCL11 to the different CXCR3 mutants is shown. IC50 values (nM) for the WT and mutants CXCR3 are also shown.