Summary of expression of FGFR3 on primary MM cells in relation to sensitivity to PRO-001
Patient no. . | FGFR3 by flow cytometry . | FGFR3 genotype . | % annexin V control . | % annexin V PRO-001, 5 μg/mL . | % increase annexin V . |
|---|---|---|---|---|---|
| 1 | ND | WT | 8.0 | 47.6 | 36.5 |
| 2 | + | WT | 12.4 | 35.7 | 20.4 |
| 3 | ++ | WT | 35.3 | 67.5 | 32.2 |
| 4 | ++ | WT | 18.2 | 98.3 | 80.0 |
| 5 | +++ | WT | 10.0 | 28.3 | 18.3 |
| 6 | - | ND | 5.0 | 10.6 | 5.6 |
| 7 | - | ND | 12.9 | 9.9 | -3.0 |
| 8 | - | ND | 23.0 | 30.0 | 7.0 |
| 9 | - | ND | 10.8 | 9.5 | -1.3 |
| 10 | - | ND | 22.3 | 24.3 | 2.0 |
Patient no. . | FGFR3 by flow cytometry . | FGFR3 genotype . | % annexin V control . | % annexin V PRO-001, 5 μg/mL . | % increase annexin V . |
|---|---|---|---|---|---|
| 1 | ND | WT | 8.0 | 47.6 | 36.5 |
| 2 | + | WT | 12.4 | 35.7 | 20.4 |
| 3 | ++ | WT | 35.3 | 67.5 | 32.2 |
| 4 | ++ | WT | 18.2 | 98.3 | 80.0 |
| 5 | +++ | WT | 10.0 | 28.3 | 18.3 |
| 6 | - | ND | 5.0 | 10.6 | 5.6 |
| 7 | - | ND | 12.9 | 9.9 | -3.0 |
| 8 | - | ND | 23.0 | 30.0 | 7.0 |
| 9 | - | ND | 10.8 | 9.5 | -1.3 |
| 10 | - | ND | 22.3 | 24.3 | 2.0 |
FGFR3 expression on CD138 primary MM cells was analyzed by flow cytometry and the fluorescence was expressed as follows: +, weak; ++ intermediate; +++ strong; -, absent. CD138 selected cells were screened for the FGFR3 mutations.
ND indicates not determined; WT, wild-type status.