Blood cell counts 8 hours after LPS
Treatment and genotype by sex and strain . | No. of mice . | Lymphocyte count, ×109/L (SD) . | Neutrophil count, ×109/L (SD) . | Monocyte count, ×109/L (SD) . | RBC count, ×1012/L (SD) . | Platelet count, ×109/L (SD) . |
|---|---|---|---|---|---|---|
| F, C57BL/6 | ||||||
| Thrombin inhibition, high-dose LPS | ||||||
| wt, saline/saline | 8 | ND | ND | ND | ND | 988 (89)† |
| wt, saline/LPS | 8 | ND | ND | ND | ND | 283 (85)† |
| wt, 5 mg/kg hirudin/LPS | 7 | ND | ND | ND | ND | 340 (88)† |
| Thrombin inhibition, low-dose LPS | ||||||
| wt, saline/LPS | 8 | 1.8 (0.7) | 0.30 (0.14) | 0.12 (0.04) | 9.7 (0.7) | 393 (65) |
| wt, 1 mg/kg hirudin/LPS | 8 | 1.4 (0.5) | 0.33 (0.12) | 0.12 (0.03) | 9.7 (0.9) | 358 (102) |
| wt, 5 mg/kg hirudin/LPS | 6 | 2.1 (1.0) | 0.26 (0.17) | 0.17 (0.09) | 7.5 (1.7)‡ | 355 (68) |
| Par2/Par4 low-dose LPS* | ||||||
| wt: no pretreatment/LPS | 6 | 1.2 (0.2) | 0.15 (0.07) | 0.05 (0.02) | 9.0 (1.0) | 469 (16) |
| ko: no pretreatment/LPS | 7 | 0.92 (0.18) | 0.10 (0.02) | 0.04 (0.02) | 8.5 (0.4) | 537 (68) |
| Thrombocytopenia low-dose LPS | ||||||
| wt, control serum/LPS | 8 | 2.0 (0.4) | 0.46 (0.12) | 0.14 (0.03) | 9.4 (0.5) | 401 (68) |
| wt, antiplatelet serum/LPS | 8 | 1.6 (0.6) | 0.28 (0.13)§ | 0.08 (0.03)‡ | 8.4 (0.7) | 187 (95)‡ |
| M, 129/C57BL/6 | ||||||
| Thrombin inhibition PAR2 littermates, low-dose LPS | ||||||
| wt, 1 mg/kg hirudin/LPS | 11 | 1.5 (0.8) | 0.39 (0.28) | 0.22 (0.09) | 9.5 (0.7) | 403 (95) |
| ko, 1 mg/kg hirudin/LPS | 11 | 1.8 (0.6) | 0.51 (0.24) | 0.29 (0.14) | 9.6 (0.3) | 358 (88) |
| Par1/Par2 high-dose LPS* | ||||||
| wt, no pretreatment/LPS | 6 | 1.1 (0.4) | 0.10 (0.08) | 0.08 (0.04) | 9.3 (0.6) | 432 (49) |
| ko, no pretreatment/LPS | 7 | 1.3 (0.5) | 0.30 (0.22) | 0.12 (0.08) | 9.7 (0.8) | 505 (193) |
| Par1/Par2 low-dose LPS | ||||||
| wt, no pretreatment/LPS | 5 | 2.8 (1.4) | 0.79 (0.48) | 0.37 (0.14) | 10.7 (1.3) | 394 (100) |
| ko, no pretreatment/LPS | 5 | 1.3 (0.5)§ | 0.41 (0.12) | 0.15 (0.06)§ | 11.2 (0.7) | 489 (71) |
| Fibrin(ogen) low-dose LPS | ||||||
| wt, no pretreatment/LPS | 5 | 2.6 (1.2) | 0.80 (0.61) | 0.36 (0.25) | 8.3 (0.7) | 294 (61) |
| ko, no pretreatment/LPS | 5 | 2.4 (1.7) | 0.45 (0.25) | 0.21 (0.01) | 8.8 (1.4) | 305 (36) |
Treatment and genotype by sex and strain . | No. of mice . | Lymphocyte count, ×109/L (SD) . | Neutrophil count, ×109/L (SD) . | Monocyte count, ×109/L (SD) . | RBC count, ×1012/L (SD) . | Platelet count, ×109/L (SD) . |
|---|---|---|---|---|---|---|
| F, C57BL/6 | ||||||
| Thrombin inhibition, high-dose LPS | ||||||
| wt, saline/saline | 8 | ND | ND | ND | ND | 988 (89)† |
| wt, saline/LPS | 8 | ND | ND | ND | ND | 283 (85)† |
| wt, 5 mg/kg hirudin/LPS | 7 | ND | ND | ND | ND | 340 (88)† |
| Thrombin inhibition, low-dose LPS | ||||||
| wt, saline/LPS | 8 | 1.8 (0.7) | 0.30 (0.14) | 0.12 (0.04) | 9.7 (0.7) | 393 (65) |
| wt, 1 mg/kg hirudin/LPS | 8 | 1.4 (0.5) | 0.33 (0.12) | 0.12 (0.03) | 9.7 (0.9) | 358 (102) |
| wt, 5 mg/kg hirudin/LPS | 6 | 2.1 (1.0) | 0.26 (0.17) | 0.17 (0.09) | 7.5 (1.7)‡ | 355 (68) |
| Par2/Par4 low-dose LPS* | ||||||
| wt: no pretreatment/LPS | 6 | 1.2 (0.2) | 0.15 (0.07) | 0.05 (0.02) | 9.0 (1.0) | 469 (16) |
| ko: no pretreatment/LPS | 7 | 0.92 (0.18) | 0.10 (0.02) | 0.04 (0.02) | 8.5 (0.4) | 537 (68) |
| Thrombocytopenia low-dose LPS | ||||||
| wt, control serum/LPS | 8 | 2.0 (0.4) | 0.46 (0.12) | 0.14 (0.03) | 9.4 (0.5) | 401 (68) |
| wt, antiplatelet serum/LPS | 8 | 1.6 (0.6) | 0.28 (0.13)§ | 0.08 (0.03)‡ | 8.4 (0.7) | 187 (95)‡ |
| M, 129/C57BL/6 | ||||||
| Thrombin inhibition PAR2 littermates, low-dose LPS | ||||||
| wt, 1 mg/kg hirudin/LPS | 11 | 1.5 (0.8) | 0.39 (0.28) | 0.22 (0.09) | 9.5 (0.7) | 403 (95) |
| ko, 1 mg/kg hirudin/LPS | 11 | 1.8 (0.6) | 0.51 (0.24) | 0.29 (0.14) | 9.6 (0.3) | 358 (88) |
| Par1/Par2 high-dose LPS* | ||||||
| wt, no pretreatment/LPS | 6 | 1.1 (0.4) | 0.10 (0.08) | 0.08 (0.04) | 9.3 (0.6) | 432 (49) |
| ko, no pretreatment/LPS | 7 | 1.3 (0.5) | 0.30 (0.22) | 0.12 (0.08) | 9.7 (0.8) | 505 (193) |
| Par1/Par2 low-dose LPS | ||||||
| wt, no pretreatment/LPS | 5 | 2.8 (1.4) | 0.79 (0.48) | 0.37 (0.14) | 10.7 (1.3) | 394 (100) |
| ko, no pretreatment/LPS | 5 | 1.3 (0.5)§ | 0.41 (0.12) | 0.15 (0.06)§ | 11.2 (0.7) | 489 (71) |
| Fibrin(ogen) low-dose LPS | ||||||
| wt, no pretreatment/LPS | 5 | 2.6 (1.2) | 0.80 (0.61) | 0.36 (0.25) | 8.3 (0.7) | 294 (61) |
| ko, no pretreatment/LPS | 5 | 2.4 (1.7) | 0.45 (0.25) | 0.21 (0.01) | 8.8 (1.4) | 305 (36) |