Table 1. The molecular identification... | American Society of Hematology

Table 1.

The molecular identification and enumeration of amino acid triplets among HLA alleles belonging to the same serologic family and cross-reactive groups for patient 15

	Position:	9	11	14	16	41	45	56	59	62	66	70	74	76	80	82	90	105	107	127	131	138	142	144	147	149	151	156	158	163	166	171	177	180	183	186	193	199	Trp
Patient	A^*0207	F	Sv	R	Gr	A	Me	G	Y	Ge	rKv	aHs	H	Vd	gTI	IRg	A	S	W	K	R	M	mT	tKh	W	aAh	aHv	L	A	T	Ew	Y	Et	Q	A	K	Av	A	—
Patient	A^*2402	S	Sv	R	Gr	A	Me	G	Y	Ee	gKv	aHs	D	En	RIa	aLr	A	S	G	K	R	M	mI	tKr	W	aAh	aHv	Q	A	T	Dg	Y	Et	Q	P	K	Pi	A	—
Patient	B^*0705	Y	Sv	R	Gr	A	Ee	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	R	T	tI	tQr	W	aAr	aRe	R	A	E	Ew	Y	Dk	E	P	K	Pi	A	—
Patient	B^*1502	Y	Am	R	Gr	A	Ma	G	Y	Rn	qIs	tNt	Y	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRe	L	A	L	Ew	Y	Et	Q	P	K	Pi	A	—
B7 CREG	B^*5501	Y	Am	R	Gr	A	Ee	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRe	L	A	T	Ew	Y	Et	Q	P	K	Pi	A	0
B7 CREG	B^*5601	Y	Am	R	Gr	A	Ee	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	A	L	Ew	Y	Et	Q	P	K	Pi	A	1
B7 CREG	B^*5401	Y	Am	R	Gr	A	Ge	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	A	T	Ew	Y	Et	Q	P	K	Pi	A	2
B7 CREG	B^*4002	H	Sv	R	Gr	T	Ke	G	Y	Re	qIs	tNt	Y	Es	rNI	IRg	A	P	G	N	R	T	tI	tQr	W	aAr	aRv	L	A	E	Ew	Y	Et	Q	P	K	Pi	A	5
B7 CREG	B^*4001	H	Am	R	Gr	T	Ke	G	Y	Re	qIs	tNt	Y	Es	rNI	IRg	A	P	G	N	R	T	tI	sQr	L	aAr	aRv	L	A	E	Ew	Y	Dk	E	P	K	Pi	A	7
B7 CREG	B^*2705	H	Sv	R	Gr	A	Ee	G	Y	Re	qIc	aKa	D	Ed	rTI	ILr	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	A	E	Ew	Y	Et	Q	P	K	Pi	A	8
Non Cross Reactive	B^*1401	Y	Sb	R	Gr	A	Ee	G	Y	Rn	qIc	tNt	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRe	L	A	T	Ew	H	Et	Q	P	K	Pi	A	2
Non Cross Reactive	B^*3801	Y	Sv	R	Gr	A	Ee	G	Y	Rn	qIc	tNt	Y	En	rla	aLr	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	T	T	Ew	Y	Et	Q	P	K	Pi	A	3

	Position:	9	11	14	16	41	45	56	59	62	66	70	74	76	80	82	90	105	107	127	131	138	142	144	147	149	151	156	158	163	166	171	177	180	183	186	193	199	Trp
Patient	A^*0207	F	Sv	R	Gr	A	Me	G	Y	Ge	rKv	aHs	H	Vd	gTI	IRg	A	S	W	K	R	M	mT	tKh	W	aAh	aHv	L	A	T	Ew	Y	Et	Q	A	K	Av	A	—
Patient	A^*2402	S	Sv	R	Gr	A	Me	G	Y	Ee	gKv	aHs	D	En	RIa	aLr	A	S	G	K	R	M	mI	tKr	W	aAh	aHv	Q	A	T	Dg	Y	Et	Q	P	K	Pi	A	—
Patient	B^*0705	Y	Sv	R	Gr	A	Ee	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	R	T	tI	tQr	W	aAr	aRe	R	A	E	Ew	Y	Dk	E	P	K	Pi	A	—
Patient	B^*1502	Y	Am	R	Gr	A	Ma	G	Y	Rn	qIs	tNt	Y	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRe	L	A	L	Ew	Y	Et	Q	P	K	Pi	A	—
B7 CREG	B^*5501	Y	Am	R	Gr	A	Ee	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRe	L	A	T	Ew	Y	Et	Q	P	K	Pi	A	0
B7 CREG	B^*5601	Y	Am	R	Gr	A	Ee	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	A	L	Ew	Y	Et	Q	P	K	Pi	A	1
B7 CREG	B^*5401	Y	Am	R	Gr	A	Ge	G	Y	Rn	qIy	aQa	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	A	T	Ew	Y	Et	Q	P	K	Pi	A	2
B7 CREG	B^*4002	H	Sv	R	Gr	T	Ke	G	Y	Re	qIs	tNt	Y	Es	rNI	IRg	A	P	G	N	R	T	tI	tQr	W	aAr	aRv	L	A	E	Ew	Y	Et	Q	P	K	Pi	A	5
B7 CREG	B^*4001	H	Am	R	Gr	T	Ke	G	Y	Re	qIs	tNt	Y	Es	rNI	IRg	A	P	G	N	R	T	tI	sQr	L	aAr	aRv	L	A	E	Ew	Y	Dk	E	P	K	Pi	A	7
B7 CREG	B^*2705	H	Sv	R	Gr	A	Ee	G	Y	Re	qIc	aKa	D	Ed	rTI	ILr	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	A	E	Ew	Y	Et	Q	P	K	Pi	A	8
Non Cross Reactive	B^*1401	Y	Sb	R	Gr	A	Ee	G	Y	Rn	qIc	tNt	D	Es	rNI	IRg	A	P	G	N	S	T	tI	tQr	W	aAr	aRe	L	A	T	Ew	H	Et	Q	P	K	Pi	A	2
Non Cross Reactive	B^*3801	Y	Sv	R	Gr	A	Ee	G	Y	Rn	qIc	tNt	Y	En	rla	aLr	A	P	G	N	S	T	tI	tQr	W	aAr	aRv	L	T	T	Ew	Y	Et	Q	P	K	Pi	A	3

The molecular identification and enumeration of amino acid triplets among HLA alleles belonging to the same serological family and cross-reactive groups for patient 15: the HLA matching algorithm is based on comparisons of linear sequences of amino acid triplets in antibody-accessible positions as motifs for potentially immunogenic epitopes. Each HLA antigen represents a distinct string of polymorphic triplets. The self-triplet repertoire for patient 15, with the HLA-A^*0207, A^*2402; B^*0705/6, B^*1502 phenotype is shown. Using HLA-B^*0705 (serologically HLA-B7) as an example, this table also shows how representative antigens of the B7 CREG are matched at the structural level. While the HLA-B22 splits B^*5501, B^*5601, and B^*5401 are well-matched, other B7-crossreactive antigens like B^*4001, B^*4002, and B^*2705 have many mismatched triplets (see text for details). Last column: Trp indicates total number of triplet mismatches between HLA-B^*0705 and each allele analyzed. The positions on top of the table represent polymorphic sites of HLA molecules accessible to antibodies. Each triplet is designated by its amino acid composition around a given position in the amino acid sequence. Amino acid residues are marked with the standard letter code; an uppercase corresponds to the residue in the numbered position, whereas the lowercase letters describe the nearest neighboring residues. For instance, the triplet 151 aRv represents an arginine (R) in position 151 with an alanine (a) in position 150 and a valine (v) in position 152. Many triplets are marked with one or two residues only, because their neighboring residues are the same on all HLA class I chains and they are, therefore, not shown.

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