Table 1. Combinatorial synergy between... | American Society of Hematology

Table 1.

Combinatorial synergy between Ly49D receptor, IL-12 and IL-18

	IL-18 dose
	Control IgG pretreatment							Anti-Ly49D (4E5) pretreatment
IL-12 dose, ng/mL	0 ng/mL	10 ng/mL	1 ng/mL	0.1 ng/mL	0 ng/mL	10 ng/mL	1 ng/mL	0.1 ng/mL
0	2	16	15	6.4	22	1 244	127.8	56.9
10	2	19 900	21 300	20 300	1544	31 400	34 300	34 500
1	8	18 400	23 100	2 364	104	32 900	30 100	29 000^*
0.1	12	17 400	18 900	0	223	27 300	27 700	25 200^*
0	—	—	—	—	20†	1 228†	113†	51†
10	—	—	—	—	1542†	11 500†	13 000†	14 200^*†
1	—	—	—	—	96†	14 500†	7 000†	26 636^*†
0.1	—	—	—	—	12†	9 900†	8 800†	25 200^*†

	IL-18 dose
	Control IgG pretreatment							Anti-Ly49D (4E5) pretreatment
IL-12 dose, ng/mL	0 ng/mL	10 ng/mL	1 ng/mL	0.1 ng/mL	0 ng/mL	10 ng/mL	1 ng/mL	0.1 ng/mL
0	2	16	15	6.4	22	1 244	127.8	56.9
10	2	19 900	21 300	20 300	1544	31 400	34 300	34 500
1	8	18 400	23 100	2 364	104	32 900	30 100	29 000^*
0.1	12	17 400	18 900	0	223	27 300	27 700	25 200^*
0	—	—	—	—	20†	1 228†	113†	51†
10	—	—	—	—	1542†	11 500†	13 000†	14 200^*†
1	—	—	—	—	96†	14 500†	7 000†	26 636^*†
0.1	—	—	—	—	12†	9 900†	8 800†	25 200^*†

Liver lymphocytes were obtained from untreated B6 mice and from mice pretreated with IL-12 or IL-18 at specified doses at 37°C for 30 minutes. Cells were washed, chilled, and coated with anti-NKRs for 20 minutes and then washed and cultured for 4 hours. Values represent pg/mL of IFNγ.

— indicates not applicable.

Dose combinations in which strong synergy was observed

†

Difference from control IgG

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