CD83 is critical for establishing HIV pol-specific CD8+ T cells from naive donors
. | Tetramer analysis . |
|---|---|
| Stimulators | HIV pol positivity, % |
| Donor 1 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | Died |
| Donor 2 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 0.02 |
| Donor 3 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 1.89 |
| Donor 4 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 0.02 |
| Donor 5 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 0.58 |
| Donor 6 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 1.58 |
. | Tetramer analysis . |
|---|---|
| Stimulators | HIV pol positivity, % |
| Donor 1 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | Died |
| Donor 2 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 0.02 |
| Donor 3 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 1.89 |
| Donor 4 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 0.02 |
| Donor 5 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 0.58 |
| Donor 6 | |
| A2/CD80 | Died |
| A2/CD80/CD83 | 1.58 |
Purified A2-positive CD8+ T cells from healthy donors were repeatedly stimulated with either A2/CD80 or A2/CD80/CD83 pulsed with HIV pol peptide in a weekly manner. IL-2 and IL-15 were added to the cultures between the stimulations every 3 days. After 8 rounds of stimulation, tetramer assays were performed to determine the pol-specific expansion of CD8+ T cells.