Decreased maturation of DC co-cultured with T cells activated in presence of anti-CD4 Ab
. | IL-12 p40 production . | CD40 expression . |
|---|---|---|
| Donor 1 | ||
| DCs alone | < 4 | 10 |
| DCs + T-cell stimulated | 1628 | 49.1 |
| DCs + T-cell stimulated + anti-CD4 Ab | 1000 | 27.4 |
| DCs + T-cell stimulated + control Ab | 2008 | 44.8 |
| Donor 2 | ||
| DCs alone | 13 | 9.7 |
| DCs + T-cell stimulated | 2453 | 49.6 |
| DCs + T-cell stimulated + anti-CD4 Ab | 809 | 2.2 |
| DCs + T-cell stimulated + control Ab | 1824 | 52.4 |
. | IL-12 p40 production . | CD40 expression . |
|---|---|---|
| Donor 1 | ||
| DCs alone | < 4 | 10 |
| DCs + T-cell stimulated | 1628 | 49.1 |
| DCs + T-cell stimulated + anti-CD4 Ab | 1000 | 27.4 |
| DCs + T-cell stimulated + control Ab | 2008 | 44.8 |
| Donor 2 | ||
| DCs alone | 13 | 9.7 |
| DCs + T-cell stimulated | 2453 | 49.6 |
| DCs + T-cell stimulated + anti-CD4 Ab | 809 | 2.2 |
| DCs + T-cell stimulated + control Ab | 1824 | 52.4 |
CD4+ T cells purified from 2 individuals were incubated with Ab against the domain 1 of the CD4 molecule or control Ab (both Ab at 10 μg/mL) or left untreated. After 1 hour, cells were washed and stimulated with anti-CD3/CD28 beads. After 24 hours, T cells were mixed with autologous monocyte-derived DCs at a ratio of 5:1, as described in the legend of Figure 1. IL-12 p40 production (expressed in pg/mL) and CD40 expression on DCs (expressed in MFI) were analyzed as described in Figure 1.