Recovery of Sca-1+ and c-Kit+ cells from catalase-treated and control cultures
. | Control . | . | . | Catalase . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
. | No. of cells . | % of total . | % of LIN- . | No. of cells . | % of total . | % of LIN- . | ||||
Total | 5 600 000 | 100.0 | — | 260 000 | 100.0 | — | ||||
Lineage negative | 140 000 | 2.5 | 100.0 | 79 248 | 30.5 | 100.0 | ||||
Sca-1+ | 840 | 0.0 | 0.6 | 40 020 | 15.5 | 50.5 | ||||
c-Kit+ | 117 320 | 2.1 | 83.8 | 72 670 | 28.0 | 91.7 | ||||
Sca-1+/c-Kit+ | 211 | 0.0 | 0.15 | 39 307 | 15.1 | 49.6 |
. | Control . | . | . | Catalase . | . | . | ||||
---|---|---|---|---|---|---|---|---|---|---|
. | No. of cells . | % of total . | % of LIN- . | No. of cells . | % of total . | % of LIN- . | ||||
Total | 5 600 000 | 100.0 | — | 260 000 | 100.0 | — | ||||
Lineage negative | 140 000 | 2.5 | 100.0 | 79 248 | 30.5 | 100.0 | ||||
Sca-1+ | 840 | 0.0 | 0.6 | 40 020 | 15.5 | 50.5 | ||||
c-Kit+ | 117 320 | 2.1 | 83.8 | 72 670 | 28.0 | 91.7 | ||||
Sca-1+/c-Kit+ | 211 | 0.0 | 0.15 | 39 307 | 15.1 | 49.6 |
The Sca-1+/c-Kit+ (catalase)/Sca-1+/c-Kit+ (control) ratio = 186.3.
— indicates not applicable.