Proportion of CD45.1 (donor-derived) cells in peripheral blood
. | Percentage of CD45.1 donor cells, mean ± SEM . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Group and no. of cells injected* . | 4 weeks after transplantation . | 8 weeks after transplantation . | 12 weeks after transplantation . | 30 weeks after transplantation . | |||
| LTBMCs, cultured 3 weeks without catalase | |||||||
| 50 000 | 2.8 ± 0.3 | 6.4 ± 0.6 | 7.0 ± 1.1 | 5.4 ± 0.6 | |||
| 20 000 | 2.8 ± 0.7 | 4.5 ± 0.4 | 4.9 ± 0.7 | 4.8 ± 0.8 | |||
| 5 000 | 1.8 ± 0.8 | 1.2 ± 0.9 | 2.1 ± 0.2 | ND | |||
| LTBMCs, cultured 3 weeks with catalase | |||||||
| 50 000 | 7.1 ± 1.8‡ | 11.3 ± 1.6‡ | 22.6 ± 6.6‡§ | 10.4 ± 1.2‡ | |||
| 20 000 | 5.4 ± 1.0 | 12.9 ± 2.2‡ | 7.1 ± 1.4 | 8.2 ± 0.8‡ | |||
| 5 000 | 2.7 ± 0.5 | 3.1 ± 0.9 | 5.3 ± 0.3‡ | ND | |||
| Sorted Sca-1+/LIN- from catalase-treated culture† | |||||||
| 20 000 | 15.3 ± 4.9 | 23.0 ± 8.0 | 23.3 ± 4.6 | ND | |||
. | Percentage of CD45.1 donor cells, mean ± SEM . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Group and no. of cells injected* . | 4 weeks after transplantation . | 8 weeks after transplantation . | 12 weeks after transplantation . | 30 weeks after transplantation . | |||
| LTBMCs, cultured 3 weeks without catalase | |||||||
| 50 000 | 2.8 ± 0.3 | 6.4 ± 0.6 | 7.0 ± 1.1 | 5.4 ± 0.6 | |||
| 20 000 | 2.8 ± 0.7 | 4.5 ± 0.4 | 4.9 ± 0.7 | 4.8 ± 0.8 | |||
| 5 000 | 1.8 ± 0.8 | 1.2 ± 0.9 | 2.1 ± 0.2 | ND | |||
| LTBMCs, cultured 3 weeks with catalase | |||||||
| 50 000 | 7.1 ± 1.8‡ | 11.3 ± 1.6‡ | 22.6 ± 6.6‡§ | 10.4 ± 1.2‡ | |||
| 20 000 | 5.4 ± 1.0 | 12.9 ± 2.2‡ | 7.1 ± 1.4 | 8.2 ± 0.8‡ | |||
| 5 000 | 2.7 ± 0.5 | 3.1 ± 0.9 | 5.3 ± 0.3‡ | ND | |||
| Sorted Sca-1+/LIN- from catalase-treated culture† | |||||||
| 20 000 | 15.3 ± 4.9 | 23.0 ± 8.0 | 23.3 ± 4.6 | ND | |||
ND indicates not done.
Each group contained 9 or 10 mice, and each sample was analyzed in duplicate. Each mouse also received 25 000 fresh syngeneic (CD45.2) bone marrow cells to assure survival after irradiation
For sorted Sca-1+/LIN- cells from catalase-treated culture, percentage of CD45.1 donor cells was measured at 3, 7, and 10 weeks after transplantation
Value differs from the catalase-free control culture, with P values less than .05
This value includes the results from a single mouse that had more than 70% donor cells. If that outlying animal is eliminated, the resultant value would be 15.5% ± 4.1%