LC reconstitution by various progenitor populations at different time points after adoptive transfer
. | . | Reconstitution of LCs and total epidermal cells by transplanted progenitors, % . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | 2 wk after transfer . | . | 3 wk after transfer . | . | 3 mo after transfer . | . | |||||
| Progenitor population . | Progenitor no, × 103 . | dEC/tEC, % average (range) . | dLC/tLC, % average (range) . | dEC/tEC, % average (range) . | dLC/tLC, % average (range) . | dEC/tEC, % average (range) . | dLC/tLC, % average (range) . | |||||
| HSCs | 3 | 7.14 (0.7-27.7) | 17.68 (7.1-35.2) | 2.58 (0.9-8.4) | 22.40 (11.5-40.2) | 2.55 (1.1-4.0) | 16.50 (3.5-23.1) | |||||
| CMPs | 10 | 0.43 (0.07-1.1) | 8.72 (1.8-14.0) | ND | ND | 0.5 (0.24-1.0) | 5.15 (3.1-6.7) | |||||
| CMPs | 30 | 0.58 (0.2-0.9) | 14.86 (4.3-29.2) | ND | ND | ND | ND | |||||
| GMPs | 40 | 0.09 (0.01-0.17) | 1.60 (0.1-4.6) | ND | ND | 0.12 (0.04-0.29) | 1.59 (1.3-2.3) | |||||
| CLPs | 10 | 0.04 (0-0.1) | 0.13 (0-0.6) | 0.07 (0.03-0.12) | 0.46 (0.1-1.2) | 0.48 (0.15-0.65) | 1.55 (0.6-2.4) | |||||
| CLPs | 30 | 0.02 (0.01-0.04) | 0.39 (0.23-0.55) | 0.11 (0.09-0.13) | 0.45 (0.38-0.52) | ND | ND | |||||
| Pro-T1 | 50 | 0.09 (0.03-0.2) | 0.55 (0-1.1) | 0.35 (0.06-0.81) | 3.44 (0.1-4.9) | ND | ND | |||||
| Pro-T2 | 60 | 0.01 (0-0.02) | 0.09 (0-0.3) | 0.09 (0.03-0.25) | 0.28 (0-1.1) | ND | ND | |||||
| Control | 0 | 0.02 (0-0.07) | 0 | 0.02 (0-0.06) | 0.04 (0-0.2) | 0.08 (0-0.18) | 0.12 (0-0.44) | |||||
. | . | Reconstitution of LCs and total epidermal cells by transplanted progenitors, % . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | 2 wk after transfer . | . | 3 wk after transfer . | . | 3 mo after transfer . | . | |||||
| Progenitor population . | Progenitor no, × 103 . | dEC/tEC, % average (range) . | dLC/tLC, % average (range) . | dEC/tEC, % average (range) . | dLC/tLC, % average (range) . | dEC/tEC, % average (range) . | dLC/tLC, % average (range) . | |||||
| HSCs | 3 | 7.14 (0.7-27.7) | 17.68 (7.1-35.2) | 2.58 (0.9-8.4) | 22.40 (11.5-40.2) | 2.55 (1.1-4.0) | 16.50 (3.5-23.1) | |||||
| CMPs | 10 | 0.43 (0.07-1.1) | 8.72 (1.8-14.0) | ND | ND | 0.5 (0.24-1.0) | 5.15 (3.1-6.7) | |||||
| CMPs | 30 | 0.58 (0.2-0.9) | 14.86 (4.3-29.2) | ND | ND | ND | ND | |||||
| GMPs | 40 | 0.09 (0.01-0.17) | 1.60 (0.1-4.6) | ND | ND | 0.12 (0.04-0.29) | 1.59 (1.3-2.3) | |||||
| CLPs | 10 | 0.04 (0-0.1) | 0.13 (0-0.6) | 0.07 (0.03-0.12) | 0.46 (0.1-1.2) | 0.48 (0.15-0.65) | 1.55 (0.6-2.4) | |||||
| CLPs | 30 | 0.02 (0.01-0.04) | 0.39 (0.23-0.55) | 0.11 (0.09-0.13) | 0.45 (0.38-0.52) | ND | ND | |||||
| Pro-T1 | 50 | 0.09 (0.03-0.2) | 0.55 (0-1.1) | 0.35 (0.06-0.81) | 3.44 (0.1-4.9) | ND | ND | |||||
| Pro-T2 | 60 | 0.01 (0-0.02) | 0.09 (0-0.3) | 0.09 (0.03-0.25) | 0.28 (0-1.1) | ND | ND | |||||
| Control | 0 | 0.02 (0-0.07) | 0 | 0.02 (0-0.06) | 0.04 (0-0.2) | 0.08 (0-0.18) | 0.12 (0-0.44) | |||||
Results are based on multicolor flow cytometric analysis of epidermal cells isolated from mice at indicated time points after progenitor transfer. Data were obtained from at least 3 mice (3 mo) or 5 mice (2 wk and 3 wk) that underwent transplantation per progenitor population, with the exception of 30 × 103 CLPs (2 mice each).
dEC/tEC indicates ratio of donor-derived (CD45.1+) epidermal cells per total (CD45.1+/-) epidermal cells (%); dLC/tLC, ratio of donor-derived LCs per total LCs (%); LCs are defined as I-Ab+/CD11c+ cells, and donor-derived LCs, as CD45.1+/I-Ab+/CD11c+ population; and ND, not determined.