Percent del(20q) cells by FISH in sorted peripheral blood cells obtained from the recipient at different time points after transplantation
Cell type . | April 1998* . | February 2001 . | February 2005 . |
|---|---|---|---|
| CD33+/CD14- granulocytes | 10 | 56.5 | 73 |
| CD14+/CD33++ monocytes | 11 | 60.5 | 66 |
| CD3+ T cells | 3 | 6.5 | 16 |
| CD19+ B cells | ND | 15 | 26 |
| Healthy control | 2 | 1.75 | 2 |
Cell type . | April 1998* . | February 2001 . | February 2005 . |
|---|---|---|---|
| CD33+/CD14- granulocytes | 10 | 56.5 | 73 |
| CD14+/CD33++ monocytes | 11 | 60.5 | 66 |
| CD3+ T cells | 3 | 6.5 | 16 |
| CD19+ B cells | ND | 15 | 26 |
| Healthy control | 2 | 1.75 | 2 |
Myeloid and lymphoid subpopulations were purified by flow-sorting cells labeled with fluorochrome-conjugated antibodies. Purity of sorted populations was 98% or more. Purified cells were further analyzed by fluorescence in situ hybridization (FISH) using a locus-specific DNA-probe for 20q12 (Vysis, Downers Grove, IL). At least 100 interphase cells per sorted population were analyzed; cells with 2 signals were counted as “normal” and cells with 1 signal, as “del(20q).” Unfractionated peripheral blood leukocytes from healthy donors served as controls. Variable number of tandem repeat (VNTR) analysis in February 2005 confirmed 100% donor signal in all lineages.
ND indicates not done.
158 days after transplantation