Engraftment after intracranial injections: human cells detected by real-time PCR for human Alu sequences
. | . | Brain section . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mouse no. . | Injected subpopulation . | A . | B . | C . | D . | E . | ||||
| 1 | RS-MSCs + SR-MSCSs | ND | ND | ND | 1489 ± 70.2* | ND | ||||
| 2 | RS-MSCs + SR-MSCSs | ND | ND | ND | 849 ± 80.5† | 360 ± 7.5 | ||||
| 3 | RS-MSCs only | ND | ND | ND | 1646 ± 71.8 | ND | ||||
| 4 | RS-MSCs only | ND | ND | ND | 689 ± 56.2 | 623 ± 44.1 | ||||
. | . | Brain section . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mouse no. . | Injected subpopulation . | A . | B . | C . | D . | E . | ||||
| 1 | RS-MSCs + SR-MSCSs | ND | ND | ND | 1489 ± 70.2* | ND | ||||
| 2 | RS-MSCs + SR-MSCSs | ND | ND | ND | 849 ± 80.5† | 360 ± 7.5 | ||||
| 3 | RS-MSCs only | ND | ND | ND | 1646 ± 71.8 | ND | ||||
| 4 | RS-MSCs only | ND | ND | ND | 689 ± 56.2 | 623 ± 44.1 | ||||
Values and assays as in Table 1. Values indicate the average number of human MSCs ± SD. All mice were measured at 2 weeks after injection. See Figure S4B for expression of data as percent of infused MSCs.
A indicates olfactory bulb; B, olfactory cortex; C, striatum; D, hippocampus; E, cerebellum; ND, not detected with assay sensitivity to 1 human cell per 2 × 105 mouse cells.
Ratio of RS-MSCs to SR-MSCs by SNP assay 10:1
Ratio of RS-MSCs to SR-MSCs by SNP assay greater than 1000:1