Table 2.

Profiling of the response of TAMs and PECs to LPS

Average log 2 ratio
Genes
Description
Feature ID
PECs, Unt vs LPS
TAMs, Unt vs LPS
TAM-LPS vs PEC-LPS
Cytokines/chemokines/growth factors      
   Tnf  Tumor necrosis factor   IMAGE:1327679   3.57   1.62   0.20  
   Il1b  Interleukin 1 beta   IMAGE:1139544   4.94   2.18   0.14  
   Saa3  Serum amyloid A 3   IMAGE:1264951   2.85   1.78   0.21  
   Ccl3  Chemokine (C-C motif) ligand 3   IMAGE:751833   4.94   2.53   0.08  
   Ccl5  Chemokine (C-C motif) ligand 5   IMAGE:832342   4.35   4.54   2.71  
   Ccl6  Chemokine (C-C motif) ligand 6   IMAGE:1077529   4.04   0.30   0.14  
   Ccl9  Chemokine (C-C motif) ligand 9   IMAGE:1148463   2.61   0.30   0.14  
   Cxcl10  Chemokine (C-X-C motif) ligand 10   IMAGE:1446589   3.99   3.48   2.55  
   Tgfbi  Transforming growth factor, beta induced   IMAGE:734101   3.20   1.80   9.45  
   Il12b  Interleukin 12 beta*  RT-PCR   11059.75   151.70   0.01  
   Il10  Interleukin 10*  RT-PCR   4.31   12.37   2.87  
ROI/RNI metabolism      
   Sod2  Superoxide dismutase 2, mitochondrial   IMAGE:791140   2.26   1.10   0.34  
   Nos2  Nitric oxide synthase 2, inducible, macrophage   IMAGE:922250   3.57   4.11   1.16  
   Atox1  ATX1 (antioxidant protein 1) homolog 1 (yeast)   IMAGE:479066   1.74   0.69   0.83  
   Mt1  Metallothionein 1   IMAGE:1037652   3.43   0.48   1.05  
   Mt2  Metallothionein 2   IMAGE:334351   3.58   1.30   0.45  
   Lyzs  Lysozyme   IMAGE:1382758   1.52   0.32   0.22  
Surface molecules/receptors      
   Il17r  Interleukin 17 receptor   IMAGE:1139646   2.31   0.23   0.18  
   Tnfrsf1b  Tumor necrosis factor receptor superfamily, member 1b   IMAGE:437512   4.54   3.22   0.30  
   Tnfrsf5  Tumor necrosis factor receptor superfamily, member 5   IMAGE:477641   2.77   2.13   0.41  
   Ccrl2  Chemokine (C-C motif) receptor-like 2   IMAGE:442765   2.40   2.22   0.38  
   Icam1  Intercellular adhesion molecule   IMAGE:1045389   2.13   1.13   1.18  
Signaling molecules/transcription factors      
   Nfkbib  Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta   IMAGE:946105   2.47   1.55   0.75  
   Socs3  Suppressor of cytokine signaling 3   IMAGE:988726   3.71   2.14   0.30  
   Stat3  Signal transducer and activator of transcription 3   IMAGE:479013   1.43   2.05   0.68  
   Tbx6  T-box 6   IMAGE:1446422   3.27   3.32   0.46  
   Irf1  Interferon regulatory factor 1   IMAGE:1344724   1.50   0.2   0.45  
   Irf7  Interferon regulatory factor 7   IMAGE:1430219   2.54   1.61   0.62  
Proteases and their inhibitors      
   Slpi  Secretory leukocyte protease inhibitor   IMAGE:1367299   2.46   0.12   0.12  
   Timp1  Tissue inhibitor of metalloproteinase 1   IMAGE:622732   3.73   0.31   0.08  
   Mmp14
 		 Matrix metalloproteinase 14 (membrane-inserted)
 		 IMAGE:1209994
 		 2.38
 		 1.15
 		 0.90
 
Average log 2 ratio
Genes
Description
Feature ID
PECs, Unt vs LPS
TAMs, Unt vs LPS
TAM-LPS vs PEC-LPS
Cytokines/chemokines/growth factors      
   Tnf  Tumor necrosis factor   IMAGE:1327679   3.57   1.62   0.20  
   Il1b  Interleukin 1 beta   IMAGE:1139544   4.94   2.18   0.14  
   Saa3  Serum amyloid A 3   IMAGE:1264951   2.85   1.78   0.21  
   Ccl3  Chemokine (C-C motif) ligand 3   IMAGE:751833   4.94   2.53   0.08  
   Ccl5  Chemokine (C-C motif) ligand 5   IMAGE:832342   4.35   4.54   2.71  
   Ccl6  Chemokine (C-C motif) ligand 6   IMAGE:1077529   4.04   0.30   0.14  
   Ccl9  Chemokine (C-C motif) ligand 9   IMAGE:1148463   2.61   0.30   0.14  
   Cxcl10  Chemokine (C-X-C motif) ligand 10   IMAGE:1446589   3.99   3.48   2.55  
   Tgfbi  Transforming growth factor, beta induced   IMAGE:734101   3.20   1.80   9.45  
   Il12b  Interleukin 12 beta*  RT-PCR   11059.75   151.70   0.01  
   Il10  Interleukin 10*  RT-PCR   4.31   12.37   2.87  
ROI/RNI metabolism      
   Sod2  Superoxide dismutase 2, mitochondrial   IMAGE:791140   2.26   1.10   0.34  
   Nos2  Nitric oxide synthase 2, inducible, macrophage   IMAGE:922250   3.57   4.11   1.16  
   Atox1  ATX1 (antioxidant protein 1) homolog 1 (yeast)   IMAGE:479066   1.74   0.69   0.83  
   Mt1  Metallothionein 1   IMAGE:1037652   3.43   0.48   1.05  
   Mt2  Metallothionein 2   IMAGE:334351   3.58   1.30   0.45  
   Lyzs  Lysozyme   IMAGE:1382758   1.52   0.32   0.22  
Surface molecules/receptors      
   Il17r  Interleukin 17 receptor   IMAGE:1139646   2.31   0.23   0.18  
   Tnfrsf1b  Tumor necrosis factor receptor superfamily, member 1b   IMAGE:437512   4.54   3.22   0.30  
   Tnfrsf5  Tumor necrosis factor receptor superfamily, member 5   IMAGE:477641   2.77   2.13   0.41  
   Ccrl2  Chemokine (C-C motif) receptor-like 2   IMAGE:442765   2.40   2.22   0.38  
   Icam1  Intercellular adhesion molecule   IMAGE:1045389   2.13   1.13   1.18  
Signaling molecules/transcription factors      
   Nfkbib  Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta   IMAGE:946105   2.47   1.55   0.75  
   Socs3  Suppressor of cytokine signaling 3   IMAGE:988726   3.71   2.14   0.30  
   Stat3  Signal transducer and activator of transcription 3   IMAGE:479013   1.43   2.05   0.68  
   Tbx6  T-box 6   IMAGE:1446422   3.27   3.32   0.46  
   Irf1  Interferon regulatory factor 1   IMAGE:1344724   1.50   0.2   0.45  
   Irf7  Interferon regulatory factor 7   IMAGE:1430219   2.54   1.61   0.62  
Proteases and their inhibitors      
   Slpi  Secretory leukocyte protease inhibitor   IMAGE:1367299   2.46   0.12   0.12  
   Timp1  Tissue inhibitor of metalloproteinase 1   IMAGE:622732   3.73   0.31   0.08  
   Mmp14
 		 Matrix metalloproteinase 14 (membrane-inserted)
 		 IMAGE:1209994
 		 2.38
 		 1.15
 		 0.90
 

The values corresponding to each gene represents gene modulation expressed in terms of the averaged log 2 ratio of Cy5/Cy3 intensities across all the microarray experiments. Stringency limits for significant gene modulation were as follows: mean ratio of Cy5/Cy3 intensities ≥ 2.5 for up-regulated genes and ≤ 0.4 for down-regulated genes. Data shown are representative of 6 independent experiments.

*

Expression values shown for the gene checked by real-time PCR

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